胰岛素对糖尿病大鼠玻璃体及房水中E-选择素水平的影响  

Influence of insulin on E-selectin in vitreous and aqueous humor of diabetic rats

作　　者：张志红[1] 陈卫芳[2] 李令辉[1] 王改新[1] 李瑾[1] 

机构地区：[1]河北省石家庄市第二医院眼科,050051 [2]华北石油管理局总医院眼科,河北任丘062550

出　　处：《检验医学与临床》2014年第16期2203-2204,共2页Laboratory Medicine and Clinic

基　　金：河北省石家庄市科技局资助项目(131460653)

摘　　要：目的探讨胰岛素对糖尿病大鼠玻璃体及房水中E-选择素水平的影响。方法 60只大鼠分为正常对照组、糖尿病血糖未控制组、糖尿病胰岛素治疗组共3组,每组20只,喂养5个月时,应用酶联免疫吸附试验(ELISA)检测玻璃体、房水中E-选择素水平。结果各组玻璃体中E-选择素水平分别为:正常对照组(0.503±0.013)ng/mL,糖尿病血糖未控制组(0.728±0.111)ng/mL,糖尿病胰岛素治疗组(0.565±0.061)ng/mL,各组组间比较差异均有统计学意义(P<0.05)。各组房水中E-选择素水平分别为:正常对照组(0.048±0.004)ng/mL,糖尿病血糖未控制组(0.099±0.012)ng/mL,糖尿病胰岛素治疗组(0.063±0.007)ng/mL,各组间比较差异均有统计学意义(P<0.05)。结论胰岛素治疗可明显降低糖尿病鼠玻璃体及房水内E-选择素水平。Objective To explore the influence of insulin on E-selectin in vitreous and aqueous humor of dia-betic rats .Methods Sixty SD male rats were randomly divided into the following groups ：normal control（group A） , diabetic rats with uncontrolled blood glucose group （group B） ,diabetic rats insulin controlled group（group C） ,re-ceived intensive insulin therapy ,20 rats in each group .Diabetes was induced in SD rats by a single intraperitoneal in-jection of Streptozotocin（STZ） .Application of ELISA method to detect E-selectin in vitreous and aqueous humor af-ter 5 months .Results The levels of E-selectin in vitreous in group B was higher than group A and group C （P〈0 .05） ,The levels of E-selectin in vitreous respectively was group A （0 .503 ＆#177; 0 .013）ng/mL ,group B （0 .728 ＆#177; 0 .111）ng/mL ,group C （0 .565 ＆#177; 0 .061）ng/mL .Content of E-selectin（ng/mL） in aqueous humor respectively was group A （0 .048 ＆#177; 0 .004）ng/mL ,group B （0 .099 ＆#177; 0 .012）ng/mL ,group C （0 .063 ＆#177; 0 .007）ng/mL .Each group is statistically significant （P〈0 .05） .Conclusion Insulin treatment can obviously reduce the levels of E-selectin in the vitreous and aqueous humor of diabetic rat ,reduce the incidence of diabetic retinopathy .

关 键 词：胰岛素 E-选择素 糖尿病 玻璃体 房水 

分 类 号：R587.1[医药卫生—内分泌]