多耐药铜绿假单胞菌抗药基因检测及对常用消毒剂的抗性分析  

Analysis on the detection of resistance gene and resistance to common disinfectants of multidrug resistance pseudomonas aeruginosa

作　　者：栾耀芳[1] 王冠[2] 王婧男[1] 李稳[1] 张苗[1] 

机构地区：[1]山东中医药大学附属医院检验科,济南250011 [2]山东中医药大学附属医院周围血管科,济南250011

出　　处：《检验医学与临床》2014年第16期2207-2209,共3页Laboratory Medicine and Clinic

基　　金：中华医院感染控制研究基金资助项目(ZHYY 12-025)

摘　　要：目的：了解临床分离的多耐药铜绿假单胞菌携带抗药基因qacE△1-sul 1（消毒剂-磺胺复合基因）情况及对常用消毒剂抗性水平。方法采用聚合酶链反应（PCR）法和悬液定量杀菌试验分别检测临床分离的多耐药铜绿假单胞菌qacE△1-sul 1基因和对三氯异氰尿酸、聚维酮碘、戊二醛的抗性（杀灭率）。结果临床分离的10株多耐药铜绿假单胞菌中6株qacE△1-sul 1基因阳性，4株阴性。浓度为125 mg/L的三氯异氰尿酸溶液作用1 min的杀灭率为99．63％～100．00％；浓度为250 mg/L 的三氯异氰尿酸溶液作用1 min 的杀灭率为99．81％～100．00％；浓度为125、250 mg/L的三氯异氰尿酸溶液作用3、5 min的杀灭率及浓度为500 mg/L时作用1、3、5 min的杀灭率均为100．00％。有效碘浓度为125 mg/L的聚维酮碘溶液作用0．5 min的杀灭率为99．91％～100．00％，该浓度作用1、3 min的杀灭率和浓度分别为250、500 mg/L时作用0．5、1、3 min的杀灭率均达100．00％。浓度为250 mg/L的戊二醛溶液作用1 min的杀灭率为65．46％～99．75％；浓度为500 mg/L的戊二醛溶液作用1 min的杀灭率为99．84％～100．00％，这两种浓度的戊二醛溶液作用3、5 min的杀灭率均达100．00％。结论多耐药铜绿假单胞菌抗药基因qacE△1-sul 1携带率较高，本研究显示消毒剂正确运用对多耐药铜绿假单胞菌能起到较好的杀灭效果。Objective To get a general view about drug-resistant gene qacE△1-sul 1（disinfectant-sulfanila-mide compound gene） carried by multidrug resistance pseudomonas aeruginosa collected from clinic ,and the resistant level against common disinfectants .Methods PCR（polymerase chain reaction） and suspension quantitative germicidal test were selected to study resistance （killing rate） of gene qacE △1-sul 1 carried by multidrug resistance pseudo-monas aeruginosa to trichloroisocyanuric acid ,iodophor ,and glutaraldehyde .Results 6 of 10 strains of multidrug re-sistance pseudomonas aeruginosa collected from clinic were qacE △1-sul 1 positive strains .The others were negative . The killing rate of 125 mg/L trichloroisocyanuric acid affected 1 minute was 99 .63% -100 .00% .The killing rate of 250 mg/L trichloroisocyanuric acid affected 1 minute was 99 .81% -100 .00% .The killing rate of the two solutions above affected 3 minutes and 5 minutes and the solution of 500 mg/L affected 1 ,3 ,5 minutes were all 100% .The kill-ing rate of solutions with effective iodine content of 125 mg/L affected 0 .5 minute was 99 .91% -100 .00% .The so-lution above affected 1 minute and 3minute along with the 250 mg/L and 500 mg/L solutions affected 0 .5 ,1 ,3 mi-nutes ,the killing rate were all 100% .The killing rate of 250 mg/L glutaraldehyde affected 1 minute was 65 .46% -99 .75% ,that of 500 mg/L solution affected 1minute was 99 .84% -100 .00% .Both of the solutions above affected 3 minuted and 5 minutes were 100% effective .Conclusion There′s a high carrying rate of gene qacE△1-sul 1 by mul-tidrug resistance pseudomonas aeruginosa .The research shows the proper use of the disinfectants can receive a good result of killing multidrug resistance pseudomonas aeruginosa .

关 键 词：多耐药铜绿假单胞菌 qacE△1-sul 1 悬液定量杀菌试验 杀灭率 

分 类 号：R978.1[医药卫生—药品]