恶性疟原虫抗原对健康人外周血T细胞免疫功能的影响  

Immune response of purified Plasmodium falciparum antigen on human peripheral blood T lymphocytes in vitro

作　　者：陈立[1] 陶莉[1] 李倩[1] 陶志勇[1] 方强[1] 夏惠[1] 

机构地区：[1]蚌埠医学院病原生物学教研室安徽省感染与免疫重点实验室,蚌埠233030

出　　处：《中国血吸虫病防治杂志》2014年第4期410-414,共5页Chinese Journal of Schistosomiasis Control

基　　金：蚌埠医学院研究生科研创新计划资助项目(Byy.cx1304);安徽省教育厅自然科学研究重点项目(KJ2012.A.200)

摘　　要：目的探讨恶性疟原虫抗原对健康人外周血T淋巴细胞免疫功能的影响。方法健康成人外周血单个核细胞(PBMC)体外分别用恶性疟原虫(P.f)抗原(5μg/ml)和正常红细胞(nRBC)抗原(5μg/ml)进行刺激,同时给予IL-2维持细胞增殖,另外设立只加IL-2的阴性对照组进行培养。培养12 d时,刺激组细胞再用对应的抗原刺激20 h,用流式细胞仪检测T淋巴细胞亚群分泌IL-4和IFN-γ的情况,并用羧基荧光素乙酰乙酸琥珀酰亚胺酯(Carboxyfluorescein diacetate,succinimidyl ester,CFSE)标记法检测T细胞增殖反应。结果健康人PBMC经P.f抗原刺激扩增后CD4+T细胞的增殖指数(PI)明显高于nRBC抗原刺激组和阴性对照组(P均<0.05),但3组γδT的PI差异无统计学意义(P>0.05)。P.f抗原组分泌IL-4的CD4+T细胞百分率明显高于nRBC抗原组和阴性对照组(P均<0.05),但3组分泌IFN-γ的CD4+T细胞百分率差异无统计学意义(P>0.05)。结论 P.f抗原在体外可刺激健康人外周血CD4+T细胞增殖活化,后者通过优先分泌IL-4而发挥免疫调节作用。Objective To explore the effects of the purified Plasmodium falciparum（P. f）antigen on the T lymphocytes of the peripheral blood of healthy people in vitro. Methods The peripheral blood mononuclear cells（PBMCs）isolated from healthy donators were cultured with purified P. f antigen（5μg/ml）and normal RBC（nRBC）antigen（5μg/ml）,in addition, the group in which only IL-2 added was set as a negative control. After cultured for 12 d,the corresponding antigens were used to re-stimulated the activated T cells in the two stimulated groups for 20 h. Then the cells were collected,the proliferations of the T cells labeled by CFSE were analyzed,and the secretion condition of IL-4 and IFN-γof the cells were detected by flow cytome-try. Results After amplification,the proliferative index（PI ）of CD4^＋T cells that were stimulated with P. f antigen was signifi-cantly higher than those in the nRBC antigen group and the negative control group（both P＆lt;0.05）,but there was no significant difference between the PIs ofγδT cells in the above three groups（P〉0.05）. Meanwhile,the percentages of the CD4^＋T cells se-creting IL-4＋in the P. f antigen group was significantly higher than those in the other two groups（both P＆lt;0.05）,while the per-centages of T cells secreting IFN-γin the 3 groups had no significant difference（P〉0.05）. Conclusion The purified P. f anti-gen could effectively stimulate the proliferation of CD4^＋T cells in peripheral blood of healthy people,and the latter can play a role in immunoregulation through secreting IL-4 preferentially.

关 键 词：恶性疟原虫 抗原 免疫功能 T细胞 IL-4 IFN-Γ 

分 类 号：R382.31[医药卫生—医学寄生虫学]