粉尘螨3类变应原的T细胞表位预测及鉴定  

Prediction and identification of T-cell epitopes in major group 3 allergen derived from Dermatophagoides farina

作　　者：李娜[1] 李朝品[1] 刁吉东[1] 赵蓓蓓[1] 姜玉新[2] 

机构地区：[1]皖南医学院医学寄生虫学教研室,芜湖241002 [2]皖南医学院生理学教研室

出　　处：《中国血吸虫病防治杂志》2014年第4期415-419,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81270091;30872367;81172790)

摘　　要：目的预测并鉴定粉尘螨3类变应原(Der f 3)的T细胞表位。方法运用生物信息学分析软件预测Der f 3的T细胞抗原表位,并人工合成所预测的T细胞表位肽。采用改良MTT法,用预测表位肽刺激致敏鼠脾淋巴细胞进行脾淋巴细胞增殖试验;酶联免疫吸附试验检测脾细胞培养上清液中白细胞介素-2(IL-2)、γ干扰素(IFN-γ)、IL-4及IL-5水平。结果成功预测了Der f 3的5个T细胞表位肽,通过脾淋巴细胞增殖试验,其中3个表位肽序列可促进脾淋巴细胞增殖并刺激细胞因子IL-2和IFN-γ的分泌,抑制细胞因子IL-4和IL-5的分泌。其序列分别为37GDCPYQISLQSSSHFCGG54、98IYQHENYDSMTIDNDVALIKLKTPMT123和164SELQRVDIDVVSREQCDQLYS184。结论初步鉴定了Der f 3变应原中3个T细胞表位序列,为后续过敏性哮喘的诊断和特异性免疫治疗奠定基础。Objective To predict and identify T cell epitopes of major group 3 allergen derived from Dermatophagoides fari-na（Der f 3）. Methods The T cell epitopes of Der f 3 were analyzed through the sequence analysis by using the bioinformatics online tools. The five predicted peptides of T-cell epitopes were artificially synthesized. The spleen lymphocytes were co-cultured with the five T cell epitopes by using the modified MTT method and the levels of IL-2,IFN-γ,IL-4 and IL-5 in the supernatant of the cultures were detected by ELISA. Results Five T cell epitopes of Der f 3 were predicted and three of which could pro-mote the proliferation of the mouse spleen lymphocytes. The secretions of IL-2 and IFN-γwere significantly induced and the se-cretions of IL-4 and IL-5 were significantly decreased by three of five prediction epitopes of Der f 3：37GDCPYQISLQSSSHFC-GG54,98IYQHENYDSMTIDNDVALIKLKTPMT123 and 164SELQRVDIDVVSREQCDQLYS184. Conclusion Three T cell epitopes of Der f 3 have been initially identified,which lays the foundation of the diagnosis and treatment of asthma.

关 键 词：粉尘螨 粉尘螨3类变应原 T细胞表位 表位预测 脾淋巴细胞增殖试验 

分 类 号：R384.4[医药卫生—医学寄生虫学]