影响高粱茎秆汁液含量基因的精细定位(英文)  

Identification and fine mapping of the gene associated with moisture content of stem in sorghum[Sorghum bicolor(L.) Moench]

作　　者：翟国伟[1] 邹桂花[1] 严松[2] 王华[1] 邵健丰[1] 陶跃之[1] 

机构地区：[1]浙江省植物有害生物防控重点实验室,浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [2]江西省农业科学院水稻研究所,江西南昌330200

出　　处：《浙江农业学报》2014年第4期856-861,共6页Acta Agriculturae Zhejiangensis

基　　金：National Natural Science Foundation of China(31000739);Key Subject Construction Program of Zhejiang for Modern Agricultural Biotechnology and Crop Disease Control;Special Project for Key Lab of Zhejiang Academy of Agricultural Sciences

摘　　要：无论是用作牲畜饲料或榨汁做糖,茎秆汁液含量对于甜高粱而言都是一个非常重要的农艺性状。本研究通过对两套独立的F2群体观察统计发现,影响高粱茎秆汁液含量(MCS)的基因Dd呈现质量性状基因的遗传规律,且干髓对多汁为显性。用SSR标记初定位表明该基因位于第6号染色体,扩大定位群体后,用群体F2-1将其定位在标记sm06068和sm06093之间,用群体F2-2将其定位在sm06068和sm06069之间,两个区段的物理距离均约为470 kb。为了进一步缩小定位区间,对定位群体的亲本进行了测序分析并开发了2个InDel标记。最终,目标基因被锁定在只包含6个候选基因的区间范围内。基因d的精细定位为该基因的克隆及今后分子育种中的应用打下基础。The moisture content is very important for both forage and syrup use of sweet sorghum .In the present study, the gene Dd, which is related to moisture content of stem （ MCS）, was proved as qualitative and the dominance of pithy over juicy type by the genetic analysis , using two F 2 populations derived from a pithy cultivar Fangshannian crossing a juicy cultivar Shuangli and a juicy cultivar Btx 623 crossing a pithy cultivar 7361 .Primary mapping of the gene indicated that d was located on chromosome 6.By enlarging the mapping population , the locus was then fine mapped to a 470 kb interval between SSR markers sm 06068 and sm06093 for F2-1 and sm06068 and sm06069 for F2-2.To narrow down the number of candidate genes , the target regions of Fangshannian , Shuangli and 7361 were sequenced and 2 InDel markers were mined .Finally, the locus was located to the region between markers sm06068 and Sb6-2, which harbored only six candidate genes .Genetic mapping of d provided an opportunity to identify genes involved in the moisture content of sorghum .

关 键 词：高粱 基因Dd 遗传分析 精细定位 

分 类 号：Q781[生物学—分子生物学]