青花菜种质资源遗传多样性的ISSR分析  

Genetic diversity of Brassica oleracea var. italica germplasm resources revealed by ISSR analysis

作　　者：王岚[1] 朱熠鹏 蒋明[1] 楼潇潇 赵罗鹏 朱柯柯 管铭[1] 

机构地区：[1]台州学院生命科学学院,浙江椒江318000 [2]贵阳市科技干部培训中心,贵州贵阳550000

出　　处：《浙江农业学报》2014年第4期915-919,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省公益性技术应用研究计划项目(2012C32011);台州市科技计划项目(121KY16);浙江省自然科学基金项目(LY13C150003)

摘　　要：利用ISSR分子标记技术,对来自国内外的33个青花菜(Brassica oleracea var.italica)材料进行遗传多样性研究。从100条通用ISSR引物中筛选出15条用于PCR扩增,共得到可重复的211条清晰谱带,其中多态性谱带180条,多态性条带比率(PPB)为85.31%,平均Nei's基因多样性指数与Shannon's信息指数为0.332 7和0.487 7;材料间遗传距离范围为0.09～0.45,平均0.28;聚类分析表明,33份青花菜种质可分为4组,没有明显的地域分布规律。结果表明,ISSR标记可用于青花菜种质的分子鉴定;青花菜供试材料具有一定的遗传多样性。The genetic diversity of 33 broccoli （ Brassica oleracea var.italica） germplasm resources , from home and abroad, was investigated using ISSR molecular marker method .Fifteen primers were selected from 100 universal ones , and they produced 211 distinctive bands , including 180 polymorphic sites with 85.31%of polymorphic bands . The average Nei＇s genetic diversity index and Shannon＇s information index were 0.332 7 and 0.487 7 , respectively . The genetic distance ranged from 0.09 to 0.45 with an average value of 0.28 .Cluster analysis divided the 33 broccoli materials into 4 groups, and showed no obvious correlation with geographic distribution .The results revealed that the ISSR markers could be used to identify broccoli germplasm resources , and the materials tested showed a certain level of genetic diversity .

关 键 词：青花菜 种质资源 遗传多样性 

分 类 号：S635.9[农业科学—蔬菜学]