BacT/ALERT 3D技术在结核杆菌耐药检测中的应用研究  

A study on application of BacT/ALERT 3D liquid culture technology on tuberculosis drug resistance detection

作　　者：黄文忠[1] 王平平[1] 吴红照[1] 周燕珍 胡真宝[1] 

机构地区：[1]浦江县疾病预防控制中心,浙江浦江322200

出　　处：《浙江预防医学》2014年第8期764-767,共4页Zhejiang Journal of Preventive Medicine

基　　金：金华市科学技术研究项目(2010-3-082)

摘　　要：目的比较BacT/ALERT 3D法与传统罗氏(L-J)比例法在耐药结核分枝杆菌检测中的差异,评价BacT/ALERT 3D快速培养系统在结核分枝杆菌耐药检测中的应用价值。方法对219份固体培养结核杆菌阳性的培养物采用BacT/ALERT 3D液体培养技术和L-J比例法同时进行药敏试验,并进行比对分析。结果 BacT/ALERT 3D法平均检出时间(8.02±3.85)d,比L-J比例法检出时间平均缩短约20 d;BacT/ALERT 3D法检出耐药菌60株,L-J比例法检出耐药菌79株,差异无统计学意义(P>0.05);以L-J比例法结果为判定标准,BacT/ALERT 3D法对INH、RFP、EMB和SM 4种抗结核药耐药检测结果符合率分别为95.43%、92.69%、95.43%和92.24%。结论与L-J比例法比较,BacT/ALERT 3D药敏检测方法对结核杆菌的耐药检出结果有较高的一致性,BacT/ALERT 3D法检出时间明显短于L-J比例法。Objective To evaluate the BacT/ALERT 3D liquid culture technology on the detection of drug resistance of Mycobacterium tuberculosis（MTB）and to compare the difference between this technology and Lowenstein -Jensen （L -J） proportion method.Methods BacT/ALERT 3D liquid culture technology and L -J proportion technology were applied to detect the drug resistance of tuberculosis from the positive cultures of 219 solid culture samples.Results The average detection time of BacT/ALERT 3D method was 8.02 ＆#177;3.85 d,which was about 20 days shorter than that of L -J proportion method.60 drug resistance strains were found using BacT/ALERT 3D technology,While 79 drug resistance strains were found using L -J proportion technology.There showed no significant difference （P〉0.05）.The compliance rate of BacT/ALERT 3D method and L -J proportion method on the anti -tuberculosis drugs INH,RFP,EMB and SMwas 95.43%,92.69%,95.43% and 92.24% respectively.Conclusion BacT/ALERT 3D liquid culture technology could detect drug resistant TB strains rapidly with high concordance with the results of L -J proportion method on anti -tuberculosis drugs.

关 键 词：结核分枝杆菌 BacT ALERT 3D L -J 比例法 耐药 

分 类 号：R521[医药卫生—内科学] R446[医药卫生—临床医学]