脂蛋白脂酶基因多态性与高血压合并肥胖人群血脂的相关性研究  

Lipoprotein lipase gene polymorphisms: associations with hypertension combined with obesity and their lipids metabolism

作　　者：何青芳[1] 王立新[1] 俞敏[1] 胡如英[1] 龚巍巍[1] 王浩[1] 张洁[1] 方乐[1] 潘劲[1] 苏丹婷[1] 梁明斌[1] 赵鸣[1] 

机构地区：[1]浙江省疾病预防控制中心,浙江杭州310051

出　　处：《浙江预防医学》2014年第8期787-790,795,共5页Zhejiang Journal of Preventive Medicine

基　　金：浙江省科技厅钱江人才计划(2013R10078);浙江省卫生厅科研基金(2013KYB053;2008A034;2007A035;2006A019)

摘　　要：目的探讨脂蛋白脂酶(LPL)基因HindⅢ酶切多态性对高血压合并肥胖及其血脂的影响。方法选取326例高血压合并肥胖者和326名健康对照者为研究对象,开展问卷调查、医学体检和血液生化项目检测,应用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术进行LPL基因HindⅢ酶切位点多态性检测,回归分析脂蛋白脂酶基因多态性与高血压合并肥胖的关系。结果 LPL基因HindⅢ位点在两组中均以H+等位基因为主。单因素分析显示:两组间基因型和等位基因频率差异无统计学意义(P<0.05);在高血压合并肥胖组中,H+H+基因型的甘油三酯(TG)、总胆固醇(TC)值比H+H-/H-H-基因型的高(P>0.05),但高密度脂蛋白胆固醇(HDLC)与H+H-/H-H-基因型相比差异无统计学意义(P>0.05)。多因素条件Logistic回归分析显示,高空腹血糖(调整OR=21.56,95%CI:7.49～62.11)、高甘油三酯(调整OR=7.51,95%CI:4.20～13.43)、低高密度脂蛋白胆固醇(调整OR=2.67,95%CI:1.53～4.66)、高尿酸(调整OR=3.36,95%CI:1.55～7.29)以及有高血压家族史(调整OR=2.07,95%CI:1.21～3.55)与高血压合并肥胖存在关联。结论LPL基因HindⅢ酶切基因型多态性仅与高血压合并肥胖者的血脂代谢存在关联,但不能确定它是高血压合并肥胖发生的独立危险因素。Objective To investigate the associations of lipoprotein lipase （LPL）gene Hind Ⅲ polymorphisms with hypertension combined with obesity and their lipids metabolism.Methods 326 subjects of hypertension combined with obesity and 326 healthy subjects were arranged to take questionnaires survey,physical examinations and blood biochemical tests.The LPL gene Hind Ⅲ polymorphisms were detected by PCR -RFLP.Regression analysis was used in this study. Results H ＋was the dominant allele in both groups.There was no significant difference among H ＋H ＋,H ＋H -, H -H -genotypes of LPL gene between the two groups （P〉0.05）.In the hypertension combined with obesity group, H ＋H ＋genotype had significantly higher triglyceride （TG）,total cholesterol （TC）levels than H ＋H -/H -H -genotypes （P〈0.05 ）while no significantly different density lipoprotein cholesterol （HDLC）level （P〉0.05 ）.The results of conditional logistic regression analysis showed that high fasting blood glucose （FBG,adjusted OR =21.56, 95%CI：7.49 ~62.1 1 ）,highTG（adjusted OR =7.5 1 ,95 % CI：4.20 ~1 3.43 ）,lowHDLC（adjusted OR =2.67 ,95% CI：1.53 ~4.66）,high uric acid （UA,adjusted OR =3.36 ,95% CI：1.55 ~7.29）and hypertension family history （adjusted OR =2.07,95% CI：1.21 ~3.55）were the main influencing factors of the hypertension combined with obesity （all P〈0.05）.Conclusion LPL Hind Ⅲ polymorphism is significantly associated with the lipids metabolism of the hypertension combined with obesity,but it is not an assured independent risk factor for hypertension combined with obesity.

关 键 词：高血压合并肥胖 脂蛋白脂酶 Hind Ⅲ 多态性 

分 类 号：R544.1[医药卫生—心血管疾病]