云杉体细胞胚萌发前的干化标记  

A Desiccation Indicator before Germination of Picea asperata Somatic Embryos

作　　者：张建伟[1] 王军辉[1] 张守攻[1] 李青粉[1] 夏燕[1] 

机构地区：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学》2014年第7期31-36,共6页Scientia Silvae Sinicae

基　　金："十二五"国家科技支撑项目"北方针叶树种高世代育种技术研究与示范"(2012BAD01B01)

摘　　要：针对云杉体细胞胚萌发率极低和萌发质量不高的问题,以高同步化体细胞胚为材料,对干化方式、干化时间和干化普遍性等影响干化处理的因素进行探讨,并建立基于形态和颜色识别的云杉体细胞胚的干化标记。结果表明:采用"滤纸容器法"的干化方式更适用于云杉体细胞胚的干化处理;干化处理2周后能够获得最高的萌发率;本试验提供的干化处理方案对萌发促进作用具有普遍的适用性;以健壮的绿色子叶、胚轴及红色胚根为标记的体细胞胚能够普遍获得高的萌发率(平均83.63%)和较好的萌发质量。In order to overcome the low germination rate and plantlet quality of Picea asperata, partial desiccation factors, such as drying approaches, drying time and universality, were investigated with highly synchronized somatic embryos, and a crucial partial desiccation indicator was established based on variation in the embryos morphology and color. The results indicated that the ＂paper vessel method＂ was more appropriate for the partial desiccation treatment, and the maximum germination rate was obtained with drying treatment for two weeks. Meanwhile, this study provided a drying treatment program which showed a general applicability for somatic embryos of P. asperata. The marked embryos of green cotyledon, hypocotyle and red radical could universally obtain high germination rate（ average 83.63% ）and good plantlet quality.

关 键 词：云杉 体细胞胚 萌发 干化标记 

分 类 号：S718.46[农业科学—林学]