RP-HPLC测定汉黄芩苷、汉黄芩素和千层纸素A的pK_a  

Determination of pK_a values of wogonoside,wogonin and oroxylin-A by RP-HPLC

作　　者：李婷婷[1] 邝国俊 霍慧灵 张蕾[1] 

机构地区：[1]广州中医药大学中药学院,广州510006 [2]南方医科大学中医药学院,广州510515

出　　处：《药物分析杂志》2014年第8期1475-1480,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金项目(30901953);国家教育部新教师基金项目(20094425120001)

摘　　要：目的:采用反相高效液相色谱法测定汉黄芩苷、汉黄芩素和千层纸素A的pKa,为该单体的剂型设计与临床应用提供重要参考。方法:基于待测成分保留因子(k)随流动相的pH变化而变化,采用乙腈-不同pH缓冲盐溶液作为流动相,依次测定汉黄芩苷、汉黄芩素和千层纸素A的k,根据pKa和k的函数关系计算其pKa。结果:汉黄芩苷、汉黄芩素和千层纸素A的pH-k曲线均呈现S型,pKa分别为3.84±0.16、7.48±0.11、7.53±0.13。结论:该方法具有样品损耗少,分析速度快,重现性好,准确度高,方便简单的特点,可用于测定汉黄芩苷、汉黄芩素和千层纸素A的pKa。Objective： To apply the RP- HPLC method for determination of the pKavalues of wogonoside,wogonin and oroxylin- A,in order to provide the reference to the rational design of dosage forms and clinical application of the drug. Methods： Acetonitrile- different pH buffer solutions were used as the mobile phase to determinate the k values of wogonoside,wogonin and orgxylin- A on the basis of the relationship of retention factor（ k） of the compound investigated and the pH value of the mobile phase. k was determined respectively for wogonoside,wogonin and orgxylin- A and the pKavalue was calculated according to the formula of pKaand k. Results： The pH- k plot of wogonoside,wogonin and oroxylin- A was a sigmoid curve and the pKavalue was 3. 84 ± 0. 16,7. 48 ± 0. 11 and7. 53 ± 0. 13 respectively. Conclusion： The proposed method is low sample- consuming,with characteristics of rapid sample analysis,high sensitivity and accuracy,and convenience which could be easily used for analysis of the pKa values of wogonoside,wogonin and oroxylin- A.

关 键 词：反相高效液相色谱 汉黄芩苷 汉黄芩素 千层纸素A 弱酸离解常数 理化参数 PKA 

分 类 号：R917[医药卫生—药物分析学]