棉油皂脚中棉籽糖的提取纯化及HPLC-ELSD含量测定  

Isolation, purification and HPLC-ELSD analysis of raffinose in cottonseed soapstock

作　　者：贾蔓箐[1] 唐辉[2] 邓喜玲[2] 刘媛[2] 刘怡娟[2] 

机构地区：[1]新疆医科大学第二附属医院药剂科,乌鲁木齐830000 [2]新疆特种植物药资源教育部重点实验室,石河子大学药学院,石河子832000

出　　处：《食品科技》2014年第8期187-190,共4页Food Science and Technology

基　　金：新疆建设兵团博士基金项目(2009JC14);石河子大学重大科技攻关计划项目(gxjs2010-zdgg04)

摘　　要：从棉油皂脚中分离纯化棉籽糖,并进行准确的含量测定。样品经热水提取,Sevage法去蛋白、活性炭脱色,Sephadex LH-20柱层析后纯化后得到棉籽糖,采用Sugar-D色谱柱,流动相为75：25（v/v）的乙腈水混合液,使用蒸发光散射器进行分析检测。纯化后得到浅黄色的棉籽糖针状结晶,紫外光谱分析显示,在195 nm波长处有明显吸收峰,260、280 nm等处无吸收峰,说明被测物为多糖,且不含蛋白质;活性炭脱色后脱色率为90.54%;棉籽糖在5.02~40.16μg线性关系良好,平均回收率为96.62%,纯化后的棉籽糖含量显著提高。结果表明最佳提取工艺重现性较好;HPLC-ELSD法测定棉籽糖含量结果准确可靠。The process of extracting raffinose from cottonseed Soapstock has been established, and determined by HPLC-ELSD. A raffinose was isolated and purified by hot water, Sevage deproteination, activated carbon decolourization and Sephadex LH-20; the analysis was carried out on ELSD detector with with mobile phase of acetonitrile and water（95：5, v/v）. The raffinose of light yellow needle-like crystals was obtained by purification, UV-spectrophotometer analysis indicated that raffinose showed a signal of polysaccharide at 195 nm and no signals of protein, nucleic acid at 260 and 280 nm; the decolourization rate is 90.54%, there is a good linear from 5.02 μg to 40.16 μg for raffinose, the average recoveries of which is 96.62%. After purification levels of raffinose significantly improved. The optimized extraction process indicates satisfied repeatability and the method for determining phytosterols by HPLC-ELSD is accurate and reliable.

关 键 词：棉油皂脚 棉籽糖 提取 HPLC-ELSD 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]