2011年至2013年北京市海淀区羊坊店社区儿童咽扁桃体炎酿脓链球菌分子生物学特征  

Molecular biological characteristics of Streptococcus pyogenes causing children tonsillitis in Yangfangdian com- munity Haidian District of Beijing from 2011 to 2013

作　　者：梁云梅[1] 杨永弘[2] 俞桑洁[2] 袁林[2] 姚开虎[2] 任思其[1] 张金[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院儿科,100038 [2]首都医科大学附属北京儿童医院儿科研究所微生物室

出　　处：《中华实用儿科临床杂志》2014年第16期1220-1223,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的分析致北京市海淀区羊坊店社区儿童咽扁桃体炎酿脓链球菌（GAS）分子生物学特征。方法2011年4月至2013年4月首都医科大学附属北京世纪坛医院门诊诊治咽扁桃体炎患儿94例，获取17株GAS。应用多聚酶链反应（PCR）扩增编码成熟M蛋白基因（encoding mature Mprotein gene，emm gene），7对等位看家基因gki、gtr、murl、rets、recP、xpt及yqiL，超抗原speA及speC基因，大环内酯类耐药基因ermB、mefa及ermRT。对emm基因及7对等位看家基因PCR扩增产物进行测序。将所测得的emm基因序列提交到美国疾病控制中心网站，获取emm分型。将所测得的7对等位看家基因序列发送到美国多位点序列分型（MLST）网站，获取等位基因谱，以确定序列分型（ST）。对超抗原基因speA及speC和耐药基因ermB、mefA及ermRT的PCR产物进行凝胶电泳，获取相应的定性结果。结果本研究共发现emml2．0（76．4％），emml．0（5．9％），emm89．D（5．9％），emm75．0（5．9％）和emm22．0（5．9％）5种emm基因分型GAS克隆株流行；emml2．0型及其亚型GAS等位基因谱属于ST36型；emml．0型GAS等伊基因谱属于ST28型；emm89．0型GAS等位基因谱属于STl01型；emm75．0型GAS等位基因谱属于ST49型；emm22．0型GAS等位基因谱属于ST46型；其中大环内酯类耐药基因ermB的携带率为94．1％（16／17例），mefA及ermRT的携带率为0；超抗原speA基因携带率为0，超抗原speC基因携带率为100．0％（17／17例）。结论emml2．0（ST36）型GAS是2011年至2013年致北京市海淀区羊坊店社区儿童咽扁桃体炎最常见的流行克隆菌株，主要携带大环内酯类耐药基因ermB及超抗原基因speC．Objective To analyze the molecular biological characteristics of Streptococcus pyogenes （GAS） causing children tonsillitis in Yangfangdian community Haidian district, Beijing between 2011 and 2013. Methods From Apr. 2011 to Apr. 2013, there were 94 children with tonsillitis treated in outpatient of Beijing Shijitan Hospital Af- filiated to Capital Medical University, and 17 strains GAS were isolated from these pediatrician outpatients. Their enco- ding mature M protein gene （ emm gene ）, 7 pairs of alleles housekeeping gene （ gki, gtr, mud, mutS, recP, xpt and yqiL） , superantigen speA and speC genes, macrolide-resistance genes ermB, mefA and ermRT were amplified by polyme- rase chain reaction （PCR）. The PCR amplified products of emm gene and 7 pairs of alleles housekeeping gene were se- quenced. The emm gene sequences were stored in the Centers for Disease Control and Prevention database. The emm types of GAS were identified. The 7 pairs of alleles housekeeping gene sequences of GAS were sent to the multilocus se- quence typing （MLST） website for their iso-allelic sequences to identify their sequence types （ST）. The PCR amplified products of superantigen gene speA and speC, maernlide-resistance genes ermB, mefA and ermRT were performed with agar gel electrophoresis. The corresponding qualitative results were obtained. Results Five cram-types were disco- vered：emml2. 0 （ 76.4% ）, emml. 0 （ 5.9% ）, emm89. 0 （ 5.9% ） , emm75. 0 （ 5.9% ） and emm22. 0 （ 5.9% ） ; emml2.0 and its subtype strains had the same allelic profiles which belonged to ST36 type; emml. 0 strain belonged to ST28; emm89. 0 strain belonged to STlO1;emm75. 0 strain belonged to ST49; emm22. 0 strain belonged to ST46; 94.1% （ 16/17 ） strains cml-ied macrolide-resistance gene ermB. None carried macrolide-resistance genes mefA and er- toRT. None carried superantigen speA gene. 100.0% （17/17 cases） carried superantigen speC gene. Conclusions emml2.0 （ST36） strain was the most common type of GAS ca

关 键 词：咽扁桃体炎 酿脓链球菌 儿童 

分 类 号：R766.18[医药卫生—耳鼻咽喉科]