大叶性肺炎患儿血清肿瘤坏死因子-α、白细胞介素-6、白细胞介素-8、白细胞介素-10和高迁移率族蛋白B1表达意义  

Difference of serum tumor necrosis factor-α, interleukin-6, interleukin-8, interleukin-10 and high mobility group box-I among children with different infectious status of lobar pneumonia

作　　者：杜春艳[1] 卢强 李玉品[1] 杨洁[1] 王春晖[1] 田娟[1] 田姣[1] 张妮妮[1] 张良娟[1] 王宝西[1] 江逊[1] 

机构地区：[1]第四军医大学唐都医院儿科,西安710038 [2]胸外科

出　　处：《中华实用儿科临床杂志》2014年第16期1224-1226,共3页Chinese Journal of Applied Clinical Pediatrics

基　　金：陕西省自然科学基金（2012JM4022）

摘　　要：目的探讨大叶性肺炎患儿血清TNF-α、IL-6、IL-8、IL-10和高迁移率族蛋白BI（HMGBl）水平的变化及其相关性。方法采用双抗体夹心酶联免疫吸附法（ELISA）检测10例健康对照儿童、3l例轻度大叶性肺炎患儿和14例重度大叶性肺炎患儿的血清TNF-α、IL-6、IL-8、IL-10和HMGBI水平，分析不同程度肺炎患儿血清HMGBl、TNF-α、IL-6、IL-8、IL-10水平的差异，及血清中TNF-α、IL-6、IL-8、IL-10与HMGBl水平的相关性。结果重度大叶性肺炎组患儿、轻度大叶性肺炎组患儿血清TNF-α、IL-6、IL-8、IL-10和HMGBl水平均显著高于健康对照组（P均〈0．05）；重度大叶性肺炎组患儿和轻度大叶性肺炎患儿血清TNF-α、IL-6、IL-8、IL-10和HMGBl水平比较差异均有统计学意义（P均〈0．05）。TNF-α、IL-6、IL-8与HMGBl呈中度正相关关系（r=0．444、0．365、0．466，P=0．007、0．029、0．004）；IL-10与HMGBl呈中度负相关（r=-0．485，P=0．003）。结论大叶性肺炎患儿血清TNF-α、IL-6、IL-8、IL-10、HMGBl与疾病严重程度相关，而TNF-α、IL-6、IL-8、IL-10、HMGBl水平测定可为小儿大叶性肺炎病情进展监测、严重程度及疗效判定等提供参考。Objective To investigate the concentration changes and the inter-relationship of tumor necrosis factor-m（ TNF-α ）, interleukin-6 （ IL-6 ）, interleukin-8 （ IL-8 ）, interleukin-10 （ IL-10 ） and high mobility group box-1 （ HMGBI ） in serum of children with lobar pneumonia. Methods By double-antibody sandwich enzyme linked immu- nosorbent assay, the serum TNF-α, IL-6, IL-8, IL-1O, HMGB1 levels were determined in 10 healthy children, 31 chil- dren with mild lobar pneumonia and 14 children with severe lobar pneumonia. The correlations between serum TNF-α, IL-6,IL-8 ,IL-10 with HMGBI were analyzed. Results The levels of serum TNF-α,IL-6 ,IL-8 ,IL-10 and HMGBI in children with severe lobar pneumonia and children with mild lobar pneumonia were significantly higher than those in healthy children （ all P 〈 O. 05 ）. There were significant differences in TNF-α, IL-6, IL-8, IL-10 and HMGB1 between children with mild lobar pneumonia and children with severe lobar pneumonia （ all P 〈 0.05 ）. There was relationship between TNF-α, IL-6, IL-8 and HMGB1, showing moderate positive correlation, and the correlation coefficients were re- spectively 0.444,0. 365 and 0. 466 ,P = 0. 007,0. 029,0. 004 ; IL-10 and HMGB1 had moderate negative correlation, the correlation coefficient was 0. 485,P = 0.003. Conclusions For children with lobar pneumonia, serum concentration of TNF-α,IL-6,IL-8, IL-10 and HMGB1 were correlated with infectious status. To detect the serum level of TNF-α, IL-6, IL-8, IL-IO and HMGBI ,can provide some reference for clinical practice.

关 键 词：大叶性肺炎 肿瘤坏死因子-Α 白细胞介素-6 白细胞介素-8 白细胞介素-10 高迁移率族蛋白 B1 儿童 

分 类 号：R725.6[医药卫生—儿科]