高张盐水后处理对大鼠局灶性脑缺血再灌注损伤TNF-α、IL-1β、S-100β的影响  

Effects of hypertonic saline on TNF-α、IL-1β、S-100β following focal cerebral ischemia-reperfusion injury in rats

作　　者：赖永东[1] 金文香[1] 何荣芝[1] 

机构地区：[1]广州医科大学第二附属医院麻醉科,510260

出　　处：《实用医学杂志》2014年第16期2532-2534,共3页The Journal of Practical Medicine

基　　金：广东省卫生厅项目(编号:A2012264)

摘　　要：目的:研究高张盐水对大鼠局灶性脑缺血再灌注损伤后TNF-α、IL-1β、S-100β的影响,探讨高张盐水在脑缺血再灌注损伤中的作用。方法:清洁级雄性SD大鼠60只,随机分为4组。A组:假手术组;B组:缺血再灌注组;C组:4.2%NaCl组;D组:高张盐水组。采用线栓法制作局灶性脑缺血再灌注模型,恢复脑血流1.5 min时,经股静脉注射4 mL/kg的4.2%NaCl(C组)或7.5%NaCl(D组),A组和B组不给任何药物。再灌注22 h后,进行神经功能障碍评分,取缺血侧顶叶附近脑皮质测TNF-α、IL-1β、S-100β的含量。结果:经高张盐水后处理后,脑组织TNF-α、IL-1β、S-100β含量明显降低,但仍较假手术组高,C组和D组与B组比较有显著差异(P<0.05)。结论:高张盐水可减少缺血再灌注后脑组织TNF-α、IL-1β、S-100β含量。Objective To investigate the effect of hypertonic saline on TNF-а, IL-1β, S-100β level following focal cerebral ischemia-reperfusion injury in rats and explore the mechanisms involved. Methods Ninety rats were randomized equally into 4 groups,namely the pseudo-operated group （A group）,untreated IR injury group （B group）,C group （4.2% hypertonic saline） and D group （7.5% hypertonic saline）. Cerebral ischemia was induced by middle cerebral artery occlusion for 2 h followed by administration of the corresponding treatments in group C and D. After 22 h of reperfusion , TNF-а, IL-1β, S-100βexpression in the ischemic brain tissue were measured by enzyme-linked immunosorbent assay （ELISA）. Results After hypcrtonic saline treatment, the two saline-treated groups showed significant reduction in TNF-а、IL-1β、S-100β levels , but were still higher than pseudo-operated group（A group）. Compared with B group, significant difference can be seen among C and D group. Conclusion Hypcrtonic saline can reduce cerebral TNF-а、IL-1β、S-100βlevel of isehemia-reperfusion injury.

关 键 词：脑缺血再灌注损伤 高张盐水 TNF-α IL-1Β S-100Β 

分 类 号：R965[医药卫生—药理学]