顺铂诱导肝癌HepG2细胞凋亡的原子力显微镜研究  

AFM study of cisplatin-induced apoptosis on hepatocellular carcinoma HepG2 Cells

作　　者：巫青[1] 潘运龙[2] 皮江[3] 林贾颖[1] 张利国[1] 林唯栋[1] 

机构地区：[1]南方医科大学附属佛山医院普通外科,广东省佛山市528000 [2]暨南大学附属第一医院普通外科,广州市510630 [3]暨南大学生命科学技术学院纳米生物技术实验室,广州市510632

出　　处：《实用医学杂志》2014年第16期2550-2554,共5页The Journal of Practical Medicine

摘　　要：目的:应用原子力显微镜(atomic force microscope,AFM)研究顺铂对肝癌HepG2细胞的作用,在纳米级水平上分析顺铂诱导HepG2细胞凋亡的超微结构变化。方法:顺铂处理HepG2细胞24 h、48 h后,通过AFM分析细胞表面形貌和超微结构变化;同时,MTT法检测细胞的增殖抑制率,流式细胞术检测细胞凋亡率。结果:AFM检测到随着顺铂作用时间延长,HepG2细胞形变程度加深;细胞膜表面形成的凹陷和孔洞增多;细胞膜表面粒径大小、高低差(Rp-v)、平均粗糙度(Ra)、均方根粗糙度(Rq)和平均高度(Meant Ht)均显著增加;细胞增殖抑制率及凋亡率明显升高。结论:顺铂使HepG2细胞形态皱缩、细胞膜表面孔洞形成及粗糙度增加,进而诱导细胞的凋亡。Objective To study the effect of cisplatin on the hepatocellular carcinoma HepG2 cells by Atomic Force Microscopy （AFM）, then analysis the changes of ultrastructural in HepG2 cells during apoptosis induced by cisplatin at nanoscale level. Methods HepG2 cells were treated with cisplatin for 24h and 48h. The ultrastructural change of cell surface was detected by AFM , the inhibitory rate and apoptotic rate of cell were examined by MTT and fow cytometry. Results AFM images showed that with the prolongation of cisplatin-inducing, the deformation change of HepG2 cells varying degree. The cells appeared larger pore size of cell membrane, the value of cell membrane particle sizes, Rp-v, Ra, Rq and Meant Ht were significant increased. The inhibitory rate and apoptotic rate were significant increased. Conclusions Cisplatin induced shrinkage in cell morphology, pore formation and roughness increasing in cell membrane, thereby inducing apoptosis in HepG2 cells.

关 键 词：原子力显微镜 顺铂 肝癌HEPG2细胞 超微结构 细胞凋亡 

分 类 号：R96[医药卫生—药理学]