BAK、cFLIP在子宫内膜异位症中的表达及临床意义  

Expression and clinical significance of BAK and cFLIP in endometriosis

作　　者：王海燕[1] 黄守国[1] 蒙秋[1] 张静[1] 覃慧君 杜晓华[1] 毕燕玲[2] 

机构地区：[1]广东省人民医院,广东省医学科学院妇产科生殖医学,广州市510080 [2]广东省人民医院,广东省医学科学院检验科,广州市510080

出　　处：《实用医学杂志》2014年第16期2576-2579,共4页The Journal of Practical Medicine

基　　金：海口市重点科技计划项目(编号:2013-55)

摘　　要：目的：探讨凋亡相关基因BAK、cFLIP在EMs组织中的表达情况及其生物学行为的相关关系。方法：采用免疫组化的方法检测40例正常子宫内膜、40例卵巢EMs患者的在位内膜及异位内膜中的BAK、cFLIP基因蛋白的表达情况。结果：BAK、cFLIP基因蛋白在三组子宫内膜细胞都有表达;BAK基因蛋白在异位内膜组、在位内膜组及正常内膜组阳性表达率及阳性表达强度呈递增表达,而cFLIP呈递减表达,差异有显著性（P〈0.05）;分泌期正常内膜组BAK基因蛋白阳性表达率及阳性表达强度显著高于增殖期,而cFLIP则相反（P〈0.05）,在位内膜组、异位内膜组的增殖期与分泌期的BAK、cFLIP基因蛋白表达差异无显著性（P〉0.05）;异位内膜组和在位内膜组中BAK基因蛋白表达率及阳性表达强度重度组（Ⅲ~Ⅳ期）明显低于轻度组（Ⅰ~Ⅱ期）,而cFLIP的表达则相反（P〈0.05）。异位内膜组织中BAK与cFLIP表达水平呈负相关（r=-0.389,P〈0.05）。结论：BAK、cFLIP的负相互作用,参与了EMs凋亡与增殖紊乱过程,在EMs的诊断和治疗中具有重要意义。Objective To explore the association of expression of apoptosis-associated gene BAK and cFLIP with the biological behaviors in endometriosis. Methods The expression of BAK and cFLIP protein gene in eutopic and ectopic tissue samples from 40 cases with pathologically confirmed ovarian endometriosis and 40 cases with pathologically confirmed normal endometrium was detected by immunohistochemical method. Results ①The expression of BAK and cFLIP protein gene was found in three groups of different endometrial tissue. ②The expression of BAK protein gene was increased gradually in ectopic endometrial , eutopic endometrium and normal tissue and there was significantly difference between every two groups ,while cFLIP was contrary expressed （P 〈0.05）; ③In normal endometrium, The expression of BAK protein gene in secretory phase was higher than that in proliferative phase （P 〈 0.05）, while cFLIP was contrary expressed, in eutopic endometrium and ectopic endometrial tissue , The expression of BAK and cFLIP protein gene in secretive phase showed no statistically significant difierence with that in proliferative phase （P 〉 0.05）. ④The expression of BAK protein gene in severe group （Ⅲ-Ⅳ period） is lower than mild group both in eutopic or ectopic endometrial tissues,while cFLIP was contrary expressed （P 〈 0.05）. ⑤The expression of BAK and cFLIP was negatively correlated with each other in ectopic endometrium （r=-0.389,P〈 0.05）. Conclusion BAK and cFLIP was negatively expressed in EMS, which may take a part in the endometrial apoptosis and disorderly proliferation. BAK and cFLIP may play an important role in the the diagnosis and treatment of endometriosis.

关 键 词：EMS 基因 表达 BAK CFLIP 免疫组化 

分 类 号：R711.71[医药卫生—妇产科学]