ITTFA-UV分光光度法同时测定3种共存的抗生素  

Simultaneous determination of three coexisting antibiotics by UV spectrophotometry assisted with iterative target transformation factor analysis

作　　者：朱煜寒[1] 张东明[1] 唐美华[2] 张之翼[1] 石成成[1] 唐立超[1] 蔡清[1] 陈国松[1] 

机构地区：[1]南京工业大学理学院,江苏南京210009 [2]南京工业大学生物与制药工程学院,江苏南京210009

出　　处：《计算机与应用化学》2014年第8期943-946,共4页Computers and Applied Chemistry

摘　　要：用迭代目标转换因子分析法（ITTFA）辅助分光光度法不经分离同时测定了模拟样品和生化试剂实际样品中四环素、氯霉素和盐酸万古霉素的含量。当测量波长范围为（248～260）nm，波长点数为13，波长间隔为1mm，主因子数为3时计算结果最佳。模拟样中3组分的平均测出率均接近100％，生化试剂实际样品中3组分的加标回收率在96％～105％。与高效液相色谱法相比，本文方法简便快速，无需分离和添加任何分析试剂，只需对试样进行紫外．可见吸收光谱扫描，即可利用ITTFA法消除共存的其他未知组分的干扰，完成对试样中指定的多种抗生素的同时测定。Iterative target transformation factor analysis （ITTFA） algorithm was used to assist spectrophotometric determination of TC, CAP and vancomycin hydrocbloride in simulated samples and biochemical reagent samples simultaneously without separation. The best result was available while the absorbance at 13 wavelength points with interval of 1 nm in the range from （248 to 260） nm was selected in calculation, and the best main factor was 3. The mean recovery rate was nearly 100 % for the three components in simulated samples, and it was 96 %~105 % in biochemical reagent samples. Compared to HPLC, spectrophotometry with ITTFA was simple without using any reagent and separation. Specified antibiotics in samples with coexisting unknown components could be determined conveniently according to the scanning data of UV-vis spectra.

关 键 词：迭代目标转换因子分析 四环素 氯霉素 盐酸万古霉素 同时测定 

分 类 号：R978.1[医药卫生—药品]