利用优选的微卫星标记建立常用大小鼠品系的遗传监测体系  

Establishment of the Genetic Monitoring System in the Mouse and Rat by Using Selected Microsatellite Markers

作　　者：王越甲[1,2] 杨炜峰 车路平 王小珂 卢胜明 

机构地区：[1]北京维通利华实验动物技术有限公司,北京100107 [2]河北师范大学生命科学学院,石家庄050024

出　　处：《实验动物科学》2014年第3期5-9,14,共6页Laboratory Animal Science

基　　金：北京市科技计划课题<实验动物微生物及遗传检测体系的建立>课题资助(No.111100067311001)

摘　　要：目的利用微卫星位点在不同近交系小鼠、大鼠中可能具有的多态性,分别筛选出可以一次性区别5种常用近交系小鼠和3种常用近交系大鼠的微卫星引物组合,为近交系小鼠和大鼠的遗传监测分析提供高效的微卫星位点与简易可靠的鉴定条件。方法通过查询相关数据库以及文献报道,按照已有明确的微卫星实验数据记录为原则,进行比对和筛选,组成了小鼠37个和大鼠24个微卫星位点库。通过PCR扩增和5%琼脂糖电泳鉴定,优化筛选出可以区分单一品系和所有品系的微卫星位点组合。结果小鼠优选出11个位点可用于5种常用近交系的鉴定,并建立了1个包含6个微卫星位点的库可用于5种小鼠品系的遗传监测;大鼠优选出6个微卫星位点,可用于3种常用近交系的监测。结论成功建立了一套可以区分常用5种小鼠和3种大鼠品系的微卫星监测方法。Objectives To discriminate frequently-used 5 inbred mouse strains and 3 inbred rat strains respectively for genetic monitoring with easiness and credibility,we screened the combinations of the microsatellite loci with polymorphisms in those mouse and rat strains.Method According to the detected strains,by querying the database as well as reported literatures with definite records,the microsatellite data base composed of microsatellite loci panel （37 loci for mouse,24 loci for rat） were compared and screened.By using PCR amplification and 5％ agarose gel electrophoresis,microsatellite loci combination could be identified and optimized for distinguishing one strain from another.Result For mouse,11 loci was optimized to be used for identification of 5 inbred strains,and a microsatellite panel containing 6 loci was selected for genetic monitor those strains.For rat,6 microsatellite loci were optimized and were suitable for identification of 3 inbred strains.Conclusion The microsatellite monitoring system were successfully established for identification of 5mouse inbred strain and 3 rat inbred strains.

关 键 词：微卫星DNA 近交系小鼠 近交系大鼠 多态性 遗传监测 

分 类 号：R332[医药卫生—人体生理学]