大黄牡丹汤组方对急性胰腺炎模型大鼠腺泡细胞分泌功能的影响  

The Effect of Dahuang Mudan Decoction on Exocrine Function of Pancreatic Acinar Cells in Acute Pancreatitis Model Rats

作　　者：张延英[1] 舒畅 蔡兴[2,3] 吴建军[1] 张艳霞[1] 王茸[1] 李存祥[1] 康万荣[1] 王荣[1] 

机构地区：[1]甘肃中医学院/甘肃省中药药理与毒理学重点实验室/甘肃省高校中(藏)药化学与质量研究省级重点实验室,兰州730000 [2]甘肃省分析测试中心 [3]甘肃省实验动物管理委员会,兰州730000

出　　处：《实验动物科学》2014年第3期26-29,共4页Laboratory Animal Science

基　　金：甘肃省技术研究与开发专项(No.1305TCYA043)

摘　　要：目的研究大黄牡丹汤组方(RPDP)对急性胰腺炎(Acute pancreatitis,AP)模型大鼠胰腺腺泡细胞外分泌功能和腺泡细胞内钙离子浓度(FI)的影响。方法 SD大鼠灌胃RPDP以制备大黄牡丹汤组方含药血清(RPDP-S);SD大鼠分为假手术组和AP模型组,分离胰腺腺泡细胞并与RPDP-S共同孵育,测定腺泡细胞淀粉酶分泌水平和FI变化。结果 AP模型大鼠腺泡细胞分泌淀粉酶水平较假手术组显著升高(P<0.05),经RPDP-S处理的AP模型大鼠腺泡细胞分泌淀粉酶水平显著降低(P<0.05);FI随AP模型病程延长而升高(P<0.05),RPDP-S可抑制AP模型大鼠腺泡细胞内FI升高(P<0.05)。结论 RPDP通能抑制AP大鼠胰腺腺泡细胞的外分泌功能,抑制腺泡细胞内钙离子的升高,降低腺泡细胞内FI超载。Objective To study the effect of Dahuang Mudan Decoction （RPDP） on exocrine function and intracellular Ca2＋ concentration（FI） of pancreatic acinar cells in acute pancreatitis （AP） model rats.Method Dahuang Mudan Decoction was administrated to SD rats and the serum containing Dahuang Mudan Decoction （RPDP-S） wasprepared.SD rats randomly divided into three groups：the normal control group,the sham operation （SO） group and the AP group.Separate pancreatic acinar cells were isolated and co-incubated with RPDP-S,amylase secretion of pancreatic acinar cells and the change of FI were analyzed.Result Compared with the SO group,amylase secretion of pancreatic acinar cells was significantly increase in AP group（P ＜ O.05） ; and amylase secretion was significantly decrease in the pancreatic acinar cells treated with RPDP-S（P ＜ O.05）,and the FI was raise up along with the prolonged disease （P ＜ 0.05）.Conclusion RPDP can decrease intracellular Ca2＋ concentration,and inhibit the overload of FIand the exocrine function of pancreatic acinar cells.

关 键 词：大黄牡丹汤组方 急性胰腺炎 胰腺腺泡细胞 AP模型大鼠 FI超载 

分 类 号：Q95-33[生物学—动物学]