鸭不同MHC单倍型对禽流感重组疫苗免疫效果的影响  

Comparison of HI Antibody Titers of Ducks with Different MHC Haplotype Vaccinated to AIV Recombinant Vaccine

作　　者：赵丽丽[1] 杨超[1] 张晓娜[1] 廉传江[1] 文辉强[1] 柳金雄[2] 陈洪岩[1] 陈化兰[2] 韩凌霞[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所实验动物与比较医学团队,哈尔滨150001 [2]中国农业科学院哈尔滨兽医研究所动物流感与防控团队,哈尔滨150001

出　　处：《实验动物科学》2014年第3期30-32,共3页Laboratory Animal Science

基　　金：国家自然科学基金(No.31101800);国家科技支撑课题(No.2013BAK11B02)

摘　　要：目的不同品系的鸭对禽流感(AI)疫苗诱导的效价有差异,分析这种差异是否与主要组织相容性复合体(MHC)有关。方法用表达H5亚型禽流感HA基因重组鸭瘟活载体疫苗免疫抗原提呈相关转运体TAP基因等位基因型不同的4种SPF鸭,比较其血清HI动态变化。结果免疫后连续观察的9周中,B3群SPF鸭HI抗体效价始终显著高于另外3个群。结论鸭的不同MHC单倍型可能与AI疫苗的不同免疫效力相关。Objective Different species of ducks respond differently to AIV vaccination.To investigate whether the different response to AIV vaccination is related with different species of ducks with MHC genetic background.Method In this study,four haplotypes determined by TAP gene alleotypes located in MHC loci of SPF ducks were used to compare HI titers changes post vaccination with recombinant live attenuated H5 avian influenza vaccine.Result The result showed that B3 haplotype SPF ducks produced significantly higher HI antibody titers than others during the 9 wks experiment.Conclusion These results hints that duck MHC genetics may affect the immune responses to AIV vaccination.

关 键 词：禽流感疫苗 主要组织相容性复合体 SPF鸭 HI效价 

分 类 号：Q95-33[生物学—动物学]