拉布拉多犬与金毛猎犬毛色基因MC1R(R306ter)与TYRP1(Q331ter)SNP位点的检测  

Investigation of Coat Color Gene MC1R(R306ter) and TYRP1(Q331ter) SNP Loci in Labrador and Golden Retriever

作　　者：俞剑熊 张雅丽[1] 周子娟[1] 王福金[1] 王爱国[1] 王靖宇[1] 

机构地区：[1]大连医科大学实验动物中心,大连116044

出　　处：《实验动物科学》2014年第3期37-41,共5页Laboratory Animal Science

基　　金：国家自然科学基金(No.31272392)

摘　　要：目的建立犬毛色基因MC1R R306ter(c.C916T)与TYRP1 Q331ter(c.C991T)SNP位点的检测方法。方法采用PCR/测序的方法,对19只拉布拉多犬和15只金毛猎犬的MC1R基因c.C916T SNP位点及TYRP1基因c.C991T SNP位点的多态性进行检测,根据MC1R和TYRP1的多态性对犬的毛色基因型进行分析。结果 PCR/测序法能够对TYRP1基因c.C991T SNP位点进行明确和有效的检测。但MC1R基因c.C916T SNP位点的测序结果出现了特殊峰,经克隆/测序法确认该特殊峰型为杂合性SNP位点。毛色基因型分析表明,金毛猎犬的毛色基因型皆为纯合的eeBB(15只),未见其他基因型。拉布拉多犬的毛色基因型分别为:黑色犬EeBB(6只)、EeBb(2只);黄色犬eeBB(9只)、eeBb(2只)。未发现其他如EEBB和EEBb(黑色)、eebb(黄色)的毛色基因型。另外,拉布拉多犬样本中没有巧克力色犬,也未检测出其相应的Eebb和EEbb基因型。结论本研究成功建立了犬毛色基因MC1R R306ter(c.C916T)与TYRP1 Q331ter(c.C991T)SNP位点的检测方法,为分析毛色基因型与导盲犬培训成功率之间的相关性提供了前期工作基础。Objective To establish the method for detecting SNP loci of coat color gene MC1R R306ter （c.C916T） and TYRP1 Q331 ter （c.C991 T） in canine.Method In order to establish the SNP detecting method,the SNP of coat color gene MC1R c.C916T and TYRP1 c.C991T in canine were detected by using PCR/sequencing method.And then,using the established method,the genotypes of coat color genes in 19 Labrador and 15 Golden Retriever dogs were investigated.Result The established PCR/sequencing method could detect the SNP loci for TYRP1 c.C991T clearly and effectively.However,there was a specific peak in the PC R/sequencing results for MC1 R gene c.C916T SNP loci.Using cloning/sequencing method,we confirmed that the specific peak in the PCR/sequencing results of MC1R gene was heterozygous SNP loci.The genotype analysis for color genes showed that Golden Retriever dogs were all homozygous eeBB （n =15） genotype,no other genotypes were detected.For Labrador,black dogs showed EeBB （n =6） and EeBb （n =2） genotypes,yellow dogs showed eeBB （n =9） and eeBb （n =2） genotypes.No others genotypes such as EEBB and EEBb （black） or eebb （yellow） were detected.Furthermore,because there was no chocolate Labrador dog,so no corresponding Eebb or EEbb genotypes were detected.Conclusion The PCR/sequencing method for detecting the SNP loci of coat color gene MC1R R306ter （c.C916T） and TYRP1 Q331ter （c.C991T） in canine is successfully established.These works provide the basis for investigating the correlation between coat color genotypes and training success rates of guide dogs.

关 键 词：拉布拉多犬 金毛猎犬 毛色基因 单核苷酸多态性 

分 类 号：Q38[生物学—遗传学]