负压处理梯度电泳凝胶对PCR-DGGE实验结果的影响  

The Influence of Negative-pressure Process on PCR-DGGE Results

作　　者：胡日新[1] 潘立博[1] 姜新[2] 王靖宇[1] 王福金[1] 

机构地区：[1]大连医科大学实验动物中心,大连116044 [2]通化师范学院分院,通化134000

出　　处：《实验动物科学》2014年第3期46-49,52,共5页Laboratory Animal Science

摘　　要：目的比较负压处理前后变性梯度凝胶电泳(DGGE)胶凝固时间的变化,验证负压处理是否影响PCR-DGGE实验结果。方法使用两种不同引物扩增的PCR产物,在不同的负压处理前后,分别进行两种上述PCR产物的变性梯度凝胶电泳。结果 1)不同的负压处理对变性胶的凝固时间没有明显的影响;2)不同负压处理前后,对PCRDGGE的实验结果有明显的影响。结论负压处理对PCR-DGGE的实验结果有显著影响。Objective To compare gel solidification time of DGGE before and after the negative-pressure process and to explore if the change of the negative-pressure treatment of the experiment can affect the PCR-DGGE results.Method Using amplified PCR products from two different primers,run the denaturing gradient gel electrophoresis of the two products before and after different negative-pressure treatment,respectively.Result Compared with the results without the negative-pressure treatment process,the experimental results with negative-pressure treatment were significantly different,but the denaturing gel solidification time is not significantly affected after the vacuum treatment.Conclusion Negative-pressure process has significant influence on PCR-DGGE results,and it is an indispensable part of the experiment.

关 键 词：负压 梯度电泳凝胶 PCR PCR-DGGE 

分 类 号：Q95-336[生物学—动物学]