抗TNF-α抗体减轻创伤感染应激高血糖大鼠急性胰岛素抵抗的实验研究  

Experimental study on effect of anti-TNF-α antibody to alleviate the acute insulin resistance induced by stress hyperglycemia after traumatic infection

作　　者：曲卫[1] 张静[2] 姜兆顺[1] 万国峰[1] 韩晨东[1] 李霖[1] 王静[1] 李美晔[1] 张胜兰[1] 

机构地区：[1]济南军区总医院内分泌科,山东济南250031 [2]济南军区总医院干部一科,山东济南250031

出　　处：《中国医学装备》2014年第8期4-7,共4页China Medical Equipment

基　　金：中国博士后科学基金面上项目(20080441294)"抗TNF-a抗体对创伤感染应激性高血糖大鼠急性胰岛素抵抗的作用及机制"

摘　　要：目的:研究抗肿瘤坏死因子α抗体(anti-TNF-αantibody)对腹部创伤合并感染脓毒血症大鼠的急性胰岛素抵抗的影响机制。方法:建立大鼠创伤脓毒症导致高血糖的动物模型,并随机分为4组,分别给予大剂量和小剂量抗TNF-α治疗;通过血糖、血清胰岛素水平、葡萄糖耐量试验以及高胰岛素正葡萄糖钳夹试验等方法比较各组大鼠的差异。结果:采用抗TNF-α抗体治疗可显著降低术后血清胰岛素水平、葡萄糖耐量、葡萄糖输注率和肝组织Akt磷酸化水平,提高mTOR磷酸化水平。结论:抗TNF-α抗体治疗可调节肝组织胰岛素信号通路Akt和mTOR的磷酸化水平,进而减轻创伤感染应激后大鼠急性胰岛素抵抗程度。Objective：The objective of this study was to investigate the alleviating effect of TNF-α Antibody on acute insulin resistance induced by experimental stress hyperglycemia after traumatic infection.Methods：On stress hyperglycemia model of rats induced by sepsis,rats were randomly divided into groups,which were given antagonist therapy of high-dose or low-dose TNF-α.Then blood glucose,serum insulin level,glucose tolerance test and hyperinsulinemic euglycemic clamp test were used to evaluate the differences among the intervention groups.Results：Anti-TNF-α antibody could significantly reduce serum insulin levels,glucose tolerance,rate of glucose infusion and the phosphorylation level of AKt in liver tissue,meanwhile,it could also raise the phosphorylation level of mTOR.Conclusion：Therapy using Anti-TNF-α antibody could reduce the degree of acute insulin resistance after stress hyperglycemia induced by post-traumatic infection,which may adjust by the phosphorylation level of mTOR and AKt in signaling pathway of insulin metabolism.

关 键 词：应激性高血糖 大鼠 抗TNF-α抗体 胰岛素抵抗 

分 类 号：R587[医药卫生—内分泌]