大麻基因组DNA提取方法的比较与优化  

Comparison and Optimization of Different DNA Extraction Methods for Cannabis sativa L.

作　　者：张海军[1,2] 郭丽[1] 王明泽[1] 车野[1] 侯丽[1] 李泽宇[1] 王殿奎[1] 

机构地区：[1]黑龙江省农业科学院大庆分院,黑龙江大庆163316 [2]吉林省种子管理总站,吉林长春130062

出　　处：《中国麻业科学》2014年第4期188-190,209,共4页Plant Fiber Sciences in China

基　　金：国家麻类产业技术体系项目(NYCYTX-IP-S13);黑龙江省农业科技创新工程重点(青年基金)项目

摘　　要：以大麻的幼嫩叶片和风干叶片为材料,分别采用优化的SDS法、CTAB法、高盐低pH法和碱裂解法这4种不同方法提取基因组DNA,并比较提取质量和效率。结果表明,采用4种提取方法,大麻干样叶和鲜样叶均可获得清晰DNA目的条带,鲜样叶基因组DNA纯度和得率的顺序为:SDS法>CTAB法>高盐低pH法>碱裂解法,干样叶基因组DNA纯度和得率的顺序为:高盐低pH法>SDS法>CTAB法>碱裂解法,优化的SDS法适用于大麻鲜样叶基因组DNA提取,而大麻干样叶基因组DNA的提取选用优化的高盐低pH法最适宜。The objective of this study was to optimize and compare four different DNA extraction methods which were suitable to determine the efficiency of total DNA extraction in both fresh and dry leaves of Cannabis sativa L.The results showed that SDS method,CTAB method,high salt low pH method,and alkali decomposition method could all obtain clear gel electrophoresis bands.The order of purity and yield for fresh samples was SDS method ＞ CTAB method ＞ high salt low pH method ＞ alkali decomposition method.The order of purity and yield for dry samples was high salt low pH method ＞ SDS method ＞ CTAB method ＞ alkali decomposition method.The optimized SDS method was the best total DNA extraction method for fresh leaves of Cannabis sativa L.The improved high salt low pH method was more suitable for total DNA extraction for dry leaves of Cannabis sativa L.

关 键 词：大麻 DNA 提取方法 比较 优化 

分 类 号：S563.3[农业科学—作物学]