籼粳交水稻花培DH系的籼粳成分鉴定及聚类分析  

Ingredient Identification and Cluster Analysis of Anther Culture DH Lines fromIndica-japonica Rice Cross

作　　者：姚国新[1,2] 邹礼平[1,2] 段俊枝[3] 李春生[1,2] 卢磊[1,2] 

机构地区：[1]特色果蔬质量安全控制湖北省重点实验室,湖北孝感421000 [2]湖北工程学院生命科学技术学院,湖北孝感432000 [3]河南省农业科学院,河南郑州450002

出　　处：《河南农业科学》2014年第7期23-27,共5页Journal of Henan Agricultural Sciences

基　　金：湖北省教育厅科学技术重点研究项目(2013D20132704);湖北工程学院博士科研启动基金项目(HE2013003)

摘　　要：为分析籼粳交水稻花培DH系的遗传重组情况,利用籼粳分化InDel标记鉴定了Bph68s与少蘖粳(SNJ)籼粳交的94个花培DH系的籼粳指数。结果表明,DH系的籼粳指数呈正态分布,籼粳中间型材料占66.67%,偏籼、籼稻、偏粳、粳稻材料分别占12.50%、2.11%、14.58%、4.22%,典籼和典粳材料未出现。同时挑选48个SSR标记对94个DH系进行聚类。Bph68s与SNJ的籼粳交花培DH系的遗传重组丰富,产生了多个基因重组单株,多样性高。In order to analyze the genetic recombination of anther culture DH lines,the index of 94 DH lines from the indica-japonica rice cross Bph68/SNJ was identified using the specific InDel marker.The result showed that,for the distribution of indica-japonica index,the intermediate type acounted for 66.67%,while the indica-like type,indica type,japonica-cline type and japonicatype was 12.50%,2.11%,14.58% and 4.22%,respectively.There were no typical indicaand japonica types.At the same time,a cluster analysis of 94 DH lines was performed using 48 SSR markers,indicating that the genetic recombination of DH lines from the indicajaponica cross Bph68/SNJ was rich,diversity and high,and many plants with gene recombination were obtained.

关 键 词：水稻 籼粳交 花培 籼粳成分鉴定 聚类分析 

分 类 号：S511[农业科学—作物学]