4个山羊品种GDF9、BMP15、FSHR基因的多态性分析  

Polymorphism Analysis of GDF9,BMP15 and FSHRGenes in Four Goat Breeds

作　　者：孟丽娜[1] 李婷[1] 张英杰[1] 刘月琴[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000

出　　处：《河南农业科学》2014年第7期144-149,共6页Journal of Henan Agricultural Sciences

基　　金：国家现代肉羊产业技术体系建设项目(CARS-39)

摘　　要：采用RFLP和SSCP 2种方法研究了生长分化因子9(GDF9)基因、骨形态发生蛋白15(BMP15)基因和促卵泡素受体(FSHR)基因在河北绒山羊、美姑黑山羊、承德黑山羊、河南槐山羊4个山羊品种中的多态性。结果显示,在4个山羊品种中未检测到GDF9的突变位点;BMP15基因出现1处突变位点(890bp处,G→T),但未引起基因型改变;FSHR基因出现3种基因型HH、HK、KK,存在1处突变位点(1 568bp处,C→G)。河南槐山羊Hardy-Weinberg不平衡,且为中度多态。表明检测的FSHR基因在4个山羊品种中存在多态性。RFLP and SSCP were used to research the polymorphisms of GDF9 gene,BMP15 gene and FSHR gene in the four goat breeds including Hebei cashmere goat,Meigu black goat,Chengde black goat,Henan Huai goat.The results showed that the mutation site of GDF9 gene was not detected in the four goat breeds.The BMP15 gene showed a mutation at 890bp（G→T）,but did not cause genotypes changing.The FSHRgene showed three genotypes HH,HK,KK and there was a mutation at 1 568bp（C→G）.Henan Huai goat didn＇t reach to Hardy-Weinberg equilibrium status,and it displayed moderate polymorphism.The results indicated that the FSHR gene had polymorphism in the four goat breeds.

关 键 词：山羊 GDF9基因 BMP15基因 FSHR基因 多态性 

分 类 号：S827[农业科学—畜牧学]