陈术口服液质量标准研究  

Study on Quality Standard of Chenzhu Oral Liquid

作　　者：毕晓黎[1,2] 彭丽诗[2] 罗文汇 李养学 

机构地区：[1]广东省中医药工程技术研究院,广东广州510095 [2]广州中医药大学,广东广州510405

出　　处：《辽宁中医药大学学报》2014年第8期10-13,共4页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的：建立陈术口服液的质量标准。方法：采用薄层色谱法鉴别方中的陈皮、白术、薏苡仁3味药,高效液相色谱法测定方中橙皮苷的含量,采用Discovery？HS C18色谱柱,流动相为乙腈-0.05%三氟乙酸（20∶80）,检测波长为280 nm。结果：薄层色谱法可鉴别出陈皮、白术和薏苡仁,斑点清晰,分离度好,阴性无干扰;橙皮苷在0.0431~0.4310μg范围内呈良好线性关系,相关系数r=0.9999,平均回收率为101.38%,RSD=1.08%。结论：本方法操作简单、专属性强、重复性好、结果准确可靠,可用于陈术口服液的质量控制。Objective ： To establish the quality standard of Chenzhu Oral Liquid. Methods ： TLC was used for the qualitative identification of Citri Reticnlatae Pericarpium, Rhizoma Atractylodis Macrocephalae and Coicis Semen. HPLC was used to determine the contents of hesperidin. The samples were separated on the Discovery HS C18 column; the mobile phase was acetonitrile-0.05% trifluoroacetie acid （ 20 ： 80 ）and the detection wavelength was set at 280 nm. Results ： The TLC spots of Citri Reticulatae Pericarpium, Rhizoma Atractylodis Macrocephalae and Coicis Semen were clear and well-separated without interference of negative samples. The linear relationship of hesperidin was good at the range of 0.0431-0.4310 μg （ r=0.9999 ）; the average recovery rate was 101.38%, RSD=1.08%. Conclusion： The method is simple, accurate and reliable with good specificity, which can be used for the quality control of Chenzhu Oral Liquid.

关 键 词：陈术口服液 薄层色谱法 高效液相色谱法 橙皮苷 质量标准 

分 类 号：R284.2[医药卫生—中药学]