中药柚皮苷结合兔骨髓间充质干细胞治疗兔膝关节软骨缺损实验研究  

Experimental Study on Naringin Decoction Combined with Bone Marrow Mesenchymal Stromal Stem Cells in Repair of Articular Cartilage Defects in Rabbits

作　　者：李鹏洋[1] 李春根[1] 叶超[1] 曲弋[1] 陈婧[2] 朱光全 赵赫[1] 贾红荣[1] 

机构地区：[1]北京中医药大学东直门医院骨科,北京100700 [2]北京中医药大学东直门医院脑病科,北京100700

出　　处：《辽宁中医药大学学报》2014年第8期34-38,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：北京中医药大学中青年教师资助项目(JYBZZ-JS060)

摘　　要：目的:研究中药柚皮苷结合兔骨髓间充质干细胞对兔关节软骨缺损修复的影响,评价修复效果。方法:取兔骨髓间充质干细胞进行体外增殖,植入到兔膝关节软骨缺损,并以中药柚皮苷汤灌胃,并设立单纯柚皮苷汤组、单纯骨髓间充质干细胞组、单纯关节缺损组、复合应用组。于第8周后对修复组织进行形态学观察及组织学检查。结果:术后8周,在形态学观察及组织学检查上,单纯柚皮苷汤组、单纯骨髓间充质干细胞组均取得了一定的修复效果,但是复合组的缺损修复优于其他各组,差异有统计学意义。结论:兔骨髓间充质干细胞及口服中药柚皮苷汤均能修复兔膝关节软骨缺损,复合应用能促进软骨的修复,且提高修复质量。Objective ： To further verify Naringin decoction effects on the repair of rabbit articular cartilage defects using BMSCs. Methods ： Take BMSCs rabbit in vitro proliferation and implanted into rabbit articular cartilage defects by Naringin decoction feeding. Establish Naringin decoction group, BMSCs group, rabbit articular cartilage defects group, composite applications group. Morphological observation and histological examination were performed in repaired tissue at 8 weeks. Results： After 8 weeks, in morphological observation and histological examination, Nariugin decoction group and BMSCs group achieved some repair effect.However, the repair of the defect composite applications group was better than the other groups, and the difference was statistically significant. Conclusion： Results have indicated that BMSCs and Naringin decoction can repair articular cartilage defects in rabbits.Composite applications can promote cartilage repair, and improve the quality of repair.

关 键 词：骨髓间充质干细胞 关节软骨 柚皮苷 兔 

分 类 号：R284[医药卫生—中药学]