中缅树鼩NPY,POMC和CART基因部分序列进化亲缘关系比较  

Comparison of Evolutionary Relationship of Partial cDNA Sequences of NPY,POMC and CART Genes in Tupaia belangeri

作　　者：朱万龙[1] 张浩[1] 王政昆[1] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650000

出　　处：《热带生物学报》2014年第2期120-126,共7页Journal of Tropical Biology

基　　金：国家国际科技合作项目(2014DFR31040);"十二五"国家支撑计划(2014BAZ04815);国家自然科学基金资助项目(31360096)

摘　　要：神经肽Y(Neuropeptide Y,NPY)、阿片促黑色素原(Pro-opiomelanocortin,POMC)和可卡因-安他非明转录调节肽(Cocaine and amphetamine regulated transcriptpeptide,CART)是下丘脑调节能量稳态的重要神经肽,在进化中非常保守,能准确反映远缘物种间的亲缘关系。该研究通过RT-PCR扩增中缅树鼩NPY,POMC和CART基因,分别获得133 bp,276 bp和50 bp的cDNA片段,包含的开放阅读框(open reading frame,ORF)分别为131 bp,272 bp和48 bp,各自编码43,90和16个氨基酸。通过BLAST搜索,所得中缅树鼩NPY,POMC和CART基因cDNA序列与普通狨(Callithrix jacchus)、猕猴(Macaca mulatta)、苏门答腊猩猩(Pongo abelii)、智人(Homo sapiens)、东非狒狒(Papio Anubis)、小耳大婴猴(Otolemur garnettii)、白颊长臂猿(Nomascus leucogenys)、灰仓鼠(Cricetulus griseus)和褐家鼠(Rattus norveqicus)的NPY,POMC和CART氨基酸序列同源性很高(同源性均为95%)。本研究通过ML方法以NPY,POMC和CART序列构建系统进化树,发现中缅树鼩与灵长类聚为一支,揭示了中缅树鼩与灵长类较近的亲缘关系。Neuropeptide Y（NPY）,pro-opiomelanocortin （POMC） and cocaine and amphetamine regulated transcript peptide （CART） are hypothalamic neuropeptides that play essential roles in the regulation of energy homeostasis,which is highly conserved in evolution and can accurately reflect the distant genetic relationship between species.Partial cDNA sequences of Tupaia belangeri NPY,POMC and CART genes were obtained by using the RT-PCR method.The resultant partial sequences of NPY,POMC and CART were 133 bp,276 bp and 50 bp in length,respectively,which contain open reading frames （ORF） of 131 bp,272 bp and 48 bp,separately.The proteins encoding these sequences included 43 amino acids in NPY,90 amino acids in POMC and 16 amino acids in CART.A search of the BLAST database showed that the deduced sequences of NPY,POMC and CART from T.belangeri were very similar to those of Callithrix jacchus,Macaca mulatta,Pongo abelii,Homo sapiens,Papio Anubis,Otolemur garnettii,Nomascus leucogenys,Cricetulus griseus and Rattus norveqicus.A phylogenetic tree was constructed with the NPY,POMC and CART genes to determine the evolutional position of T.belangeri by using ML method.The result showed that T.belangeri has a close relationship with primates.

关 键 词：中缅树鼩 神经肽Y 阿片促黑色素原 可卡因-安他非明转录调节肽 下丘脑 

分 类 号：Q955[生物学—动物学]