巴西橡胶树树皮环氧树脂组织切片及染色技术  

Epoxy-resin Embedded Tissue Section and Staining of the Barks of Hevea brasiliensis

作　　者：徐静文[1] 廉文君[1] 曹玉鑫[1] 夏志辉[1] 黄惜[1] 

机构地区：[1]海南省热带生物资源可持续利用重点实验室/海南大学农学院,海南海口570228

出　　处：《热带生物学报》2014年第2期162-165,161,共5页Journal of Tropical Biology

基　　金：国家自然科学基金项目(31060107;31260170);教育部高等学校博士学科点专项科研基金博导类项目(20104601110003;20114601110003)

摘　　要：介绍以环氧树脂为包埋介质,不需要碘溴染色、用于光学显微镜的巴西橡胶树半薄切片技术,详述固定、脱水、渗透、包埋、聚合、切片、染色及封片各程序。利用3种不同的染料能充分区分橡胶乳管细胞、木质部、形成层、导管、次生韧皮射线、初生韧皮纤维、皮层和表皮等,还能观察到许多在石蜡切片上不容易区分的细胞及其内部结构,如树皮表皮细胞下富含的丹宁细胞和叶绿体等。该技术是对巴西橡胶树石蜡包埋切片技术的改进和扩展。Tissue sectioning and staining are basic technologies for dissecting plant tissue and organic structure. Rubber trees （Hevea brasiliensis Mall. Arg. ） are the most important commercial source of natural rubber, and they produce and store latex in laticifers in the bark. Paraffin section stained with I -Br is usually used for observation of laticifer under light microscopy. The procedures of this method are complicated and time-consuming. Therefore, a technology for tissue section with tissue embedded in epoxy resin without using I - Br staining is introduced for laticifer observation under light microscopy, including fixation, dehydration, infiltration, embedding, polymerization, sectioning, staining, and mounting. Laticifer, xylem, cambium, vessel, phloemray ra- dial, primary phloem ray fiber, cortex, and epidermis were well distinguished on the epoxy resin-embedded tissue sections by using 3 different stains. Furthermore, numerous cells and their inclusions that were scarcely observed in paraffin section, such as tannin, chloroplast, etc were also observed on the tissues embedded in epoxy resin. This technology is an improvement in paraffin-embedded tissue section under light microscopy for the bark of the rubber tree.

关 键 词：巴西橡胶树 环氧树脂 组织切片 染色 

分 类 号：Q246[生物学—细胞生物学]