姜黄素对HaCaT细胞凋亡过程中相关基因表达的影响  

Effects of curcumin on related gene expression during HaCaT cell apoptosis

作　　者：宋巍[1] 杨海波[1] 陈兰英[1] 

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036

出　　处：《癌变．畸变．突变》2014年第4期254-257,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：河南省重点科技攻关项目(092102310006);河南城建学院校内基金(2010JYB009)

摘　　要：目的:研究姜黄素对人永生化表皮HaCaT细胞凋亡过程中相关基因表达的影响。方法:通过免疫细胞化学法和免疫印迹检测姜黄素对HaCaT细胞凋亡过程中相关基因表达的影响。结果:免疫细胞化学染色结果显示,经7.5μg/mL姜黄素处理之后,与细胞凋亡相关的抑凋亡蛋白Bcl-2表达减弱,促凋亡蛋白P53、Fas、Bax表达增强。Western blot检测结果显示,经7.5μg/mL姜黄素处理之后,与细胞凋亡相关的抑凋亡蛋白Bcl-2表达下调,促凋亡蛋白P53、Fas、Bax表达上调。结论:姜黄素诱导HaCaT细胞凋亡过程中伴随着抑凋亡基因bcl-2表达的抑制和促凋亡基因p53、Bax和Fas基因的激活。由此证明姜黄素通过多种凋亡相关基因的调控,激活凋亡信号传导途径,诱使细胞凋亡。To explore the effects of curcumin on apoptosis-related gene expression in HaCaT cells. METHODS：Immunocytochemistry and Western blot were used to detect the effects of curcumin on apoptosis-related protein expression. RESULTS：Immunocytochemistry staining results showed that the expression levels of P53,Bax,Fas were upregulated significatntly while the products of Bcl-2 proteins were downregulated significantly in cells treated with 7.5 μg/mL curcumin. Western blot analysis confirmed the same results. CONCLUSION：Curcumin induced apoptosis of HaCaT cells by inhibiting expression of Bcl-2 gene and promoting the apoptosis genes p53,Bax and Fas activation. Curcumin could induce apoptosis by regulation of a variety of apoptosis-related genes to activate the apoptosis signaling pathway.

关 键 词：HACAT细胞 细胞凋亡 姜黄素 凋亡相关基因 

分 类 号：Q255[生物学—细胞生物学]