加热处理后人黑色素瘤A375细胞对NK和DC细胞免疫活性的影响  

Effects of heated A375 melanoma cells on the activities of DC and NK cells

作　　者：曲迪[1] 徐玉清[1] 韩毅[1] 崔凤[1] 李逸文[1] 

机构地区：[1]哈尔滨医科大学附属第二医院肿瘤内科,黑龙江哈尔滨150086

出　　处：《癌变．畸变．突变》2014年第4期266-269,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：黑龙江省政府攻关课题(GB05C401-03)

摘　　要：目的:研究经加热处理的人黑色素瘤细胞A375对外周血单个核细胞来源的自然杀伤(NK)细胞及树突状细胞(DC)免疫活性的影响并探讨其作用机制。方法:体外培养人外周血单个核细胞来源的NK和DC细胞,并使其与43℃水浴加热后的A375细胞共孵育24 h。应用CCK-8法测定加热和非加热组效靶比(NK∶DC∶A375)分别为1∶2∶1、3∶6∶1、6∶12∶1时NK/DC细胞的杀伤率;应用酶联免疫吸附法(ELISA)检测上述各效靶比组NK细胞培养液中γ-干扰素(γ-INF)的释放情况。结果:在每个效靶比,与非加热组比较,加热组NK/DC的杀伤率均明显提高(P<0.05),且其杀伤率随NK/DC细胞的浓度升高而升高(<0.05)。在加热组和非加热组,含有DC较不含DC组杀伤率均明显提高(P<0.05)。加热组NK细胞γ-INF释放均较非加热组明显增加(P<0.05)。结论:经加热处理后的黑色素瘤细胞A375能够刺激NK细胞分泌更多的γ-INF,明显提高NK细胞的杀伤活性,且DC在其中起着重要作用。To study the effects of the heated A375 melanoma cells on natural killer （NK） cells and dendritic cells （DC） generated from human cultured peripheral blood mononuclear cells （PBMCs） in vitro,and to explore the mechanism. METHODS：NK cells and DCs cultured from PBNCs in vitro and reacted with A375 melanoma cells in a water-bath at 43℃. Using CCK-8 kit to test the killing rate of NK cells and DCs against A375 melanoma cells at the effector∶ratio of efficacy to target（E/T） （NK∶DC∶A375） of 1∶2∶1,3∶6∶1,and 6∶12∶1. ELISA was used to measure concentrations ofγ-INF released by NK cells at the effector∶E/T （NK∶DC∶A375） of 1∶2∶1,3∶6∶1 and 6∶12∶1. RESULTS：At each E/T ,the killing rate of the heated group against A375 melanoma cells were stronger than the unheated group （P〈0.05）,and the killing rate increased as the concentrations of NK and DC increased （P〈0.05）. The concentration of γ-INF released by the heated group was also more than the unheated group（P〈0.05）. CONCLUSION：Heated A375 melanoma cells could induce NK to release moreγ-INF and significantly increased the activity of NK cells ,in which DC played an important role.

关 键 词：树突细胞 自然杀伤细胞 黑色素瘤 免疫活性 

分 类 号：R392.3[医药卫生—免疫学]