壳聚糖-左氧氟沙星长效缓释敷料的烧伤疗效研究  

Efficacy of Wound Dressing with Microspheres Containing Levofloxacin on Burns Treatment

作　　者：高萍[1] 王晓辉[1] 黄姝杰[2] 王瑛[1] 关静[2] 李毅敏[1] 陶遵威[1] 

机构地区：[1]天津市医药科学研究所,天津300020 [2]军事医学科学院卫生装备研究所,天津300161

出　　处：《生物医学工程学杂志》2014年第4期806-810,共5页Journal of Biomedical Engineering

基　　金：天津市自然科学基金重点项目资助(09JCZDJC18800)

摘　　要：本文研究壳聚糖-左氧氟沙星长效缓释敷料的皮肤刺激性和烧伤治疗效果。通过参照GB/T16886.10-2005评价该敷料的皮肤刺激性,同时制备烧伤兔模型,分为三组,空白对照组用凡士林油纱包扎,阳性对照组用纳米银创伤贴包扎,实验样品组用壳聚糖-左氧氟沙星长效缓释敷料包扎,分别于3、6、9、14、21、28d进行伤口未愈合面积测量、羟脯氨酸含量及组织病理学检查。实验结果显示该敷料原发性刺激指数为0;实验样品组和阳性对照组在6、9、14、21d时,未愈合伤口面积均小于空白对照组(P<0.05),实验样品组在9d和14d时其未愈合伤口面积显著低于阳性对照组(P<0.05);实验样品组在6、9和14d时羟脯氨酸含量显著高于阳性对照组和空白对照组(P<0.05);通过病理学观察,在21d时实验样品组最早出现附属器。由此说明壳聚糖-左氧氟沙星长效缓释敷料的皮肤刺激性极轻微,能够有效促进烧伤创面愈合。This research was aimed to find the skin irritation and burns treatment effect of wound dressing with microspheres containing levofloxacin. We used reference GB/T16886.10-2005 to evaluate the dressing skin irritation. We prepared rabbit models divided into three groups. The control group was rapped with Vaseline gauze bandage, while the positive control group was rapped with the wounds of nano-silver paste bandage. The experimental sample group was rapped with wound dressing with microspheres containing levofloxacin. We measured the wound without healing area and the hydroxyproline content at the ends of 3 d, 6 d, 9 d, 14 d, 21 d, 28 d. and meanwhile performed histopathological examination. The experimental results showed that the dressing primary irritation index was 0. The nonhealing wound area of theexperimental sample group and positive control group at the ends of 6 d, 9 d, 14 d, 21 d were less than that of the control group （P〈0.05）. The nonhealing wound area of the experimental sample group at the ends of 9 d and 14 d was significantly lower than that of the positive control group （P〈 0.05）. The hydroxyproline content of the experimental sample group at the ends of 6 d, 9 d and 14 d was significantly higher than that of the positive control group and blank control group （P〈0. 05）. The pathology observed of the experimental sample group at 21 d were the earliest appendages. The wound dressing with microspheres containing levofloxacin has minimal skin irritation, effectively promote wound healing of burn.

关 键 词：壳聚糖 氧氟沙星 迟效制剂 烧伤 皮肤刺激 羟脯氨酸 

分 类 号：R644[医药卫生—外科学] R965.3[医药卫生—临床医学]