载骨形态发生蛋白-2活性肽可降解水凝胶体内异位成骨研究  

Ectopic Osteogenesis In Vivo Using Bone Morphogenetic Protein-2 Derived Peptide Loaded Biodegradable Hydrogel

作　　者：赵晶晶[1] 方真华[1] 黄若昆[1] 肖凯[1] 李静[1] 谢鸣[1] 勘武生[1] 

机构地区：[1]华中科技大学同济医学院附属普爱医院骨科

出　　处：《生物医学工程学杂志》2014年第4期811-815,共5页Journal of Biomedical Engineering

基　　金：武汉市临床医学科研项目资助(WX14A05)

摘　　要：载骨形态发生蛋白-2(BMP-2)活性多肽P24的聚三甲基碳酸酯-(聚氧乙烯)-聚三甲基碳酸酯(PTMC11-F127-PTMC11)注射型可降解凝胶诱导大鼠体内异位成骨,测定P24体外释放曲线。将P24与不同质量浓度的PTMC11-F127-PTMC11水凝胶复合,采用二喹啉甲酸(BCA)法测定,绘制P24释放曲线。将复合P24凝胶植于大鼠骶棘肌,行组织学切片HE染色检测其异位成骨能力。PTMC11-F127-PTMC11浓度大于20%的复合凝胶其P24经突释期后可维持缓释1个月左右,满足缓释材料要求。组织学显示第6周切片可见骨小梁。载BMP-2活性多肽P24PTMC11-F127-PTMC11凝胶在体内能有效发挥其诱导成骨活性,有望成为生长因子合适载体。We investigated the development of an injectable, biodegradable hydrogel composite of poly（trimethylene carbonate）-F127-poly（trimethylene carbonate）（PTMC11-F127-PTMC11 ）loaded with bone morphogenetic protein-2 （BMP-2） derived peptide P24 for ectopic bone formation in vivo and evaluated its release kinetics in vitro. Then we evaluated P24 peptide release kinetics from different concentration of PTMC11-F127-PTMC11 hydrogel in vitro using bicinchoninic acid （BCA）assay. P24/PTMC11-F127-PTMC11 hydrogel was implanted into each rat＇s erector muscle of spine and ectopic bone formation of the implanted gel in vivo was detected by hematoxylin and eosin stain （HE）. PTMC11-F127-PTMC11 hydrogel with concentration more than 20 percent showed sustained slow release for one month after the initial burst release. Bone trabeculae surround the P24/PTMCH-F127-PTMC11 hydrogel was shown at the end of six weeks by hematoxylin and eosin stain. These results indicated that encapsulated bone morphogenetic protein （BMP-2） derived peptide P24 remained viable in vivo, thus suggesting the potential of PTMC11-F127-PT- MC11 composite hydrogels as part of a novel strategy for localized delivery of bioactive molecules.

关 键 词：骨组织工程 骨形态发生蛋白-2 水凝胶 骨诱导 

分 类 号：R318.08[医药卫生—生物医学工程]