结合肌钙蛋白检测的心脏疲劳家兔实验研究  

A Troponin Detection-combined Study of Rabbit Experiment for Evaluating Cardiac Fatigue

作　　者：严小波[1] 罗麟梅[1] 肖守中[2] 刘磊矗[1] 邓溯源[1] 张聪[1] 夏国翔 

机构地区：[1]重庆大学体育学院心脏疲劳研究室,重庆400044 [2]重庆博精医学信息研究所,重庆400044

出　　处：《生物医学工程学杂志》2014年第4期855-860,共6页Journal of Biomedical Engineering

基　　金：中央高校基本科研业务费重点项目资助(CQDXWL-2012-Z004)

摘　　要：本研究的目的是把生物化学指标肌钙蛋白与心音学指标结合起来对家兔进行心脏疲劳的综合评估,分析运动诱发的心脏疲劳和心肌损伤。选用新西兰白兔进行多轮负重游泳实验,达到力竭状态,观察和检测运动应激后第1心音对第2心音幅值比值（S1/S2）、心率（HR）、肌钙蛋白的变化趋势以及变化在24~48h后是否恢复。实验终点是完成3次力竭实验或力竭致死。所有实验家兔在静息状态下从其血液中检测出肌钙蛋白I[（0.02±0.01）ng/mL],一般说来,这是一种肌钙蛋白的生理性释放。家兔首次力竭后肌钙蛋白I和S1/S2升高,休息24h后,S1/S2、HR和肌钙蛋白I都向基线值回复,意味着经历了心脏疲劳过程。反复力竭实验能观察到家兔心脏负荷过重现象,并导致11只家兔中3只死亡,意味着心肌损伤;其肌钙蛋白的升高可用病理性释放来解释。心肌损伤的评估不能单独依据肌钙蛋白水平的高低,而须依靠综合分析。The objective of this study is to combine troponin and indicators of cardiac acoustics for synthetically evaluating cardiac fatigue of rabbits, analyzing exercise-induced cardiac fatigue （EICF） and exercise-induced cardiac damage （EICD）. New Zealand white rabbits were used to conduct a multi-step swimming experiments with load, reaching an exhaustive state for evaluating if the amplitude ratio of the first to second heart sound （S1/S2） and heart rate （HR） during the exhaustive exercise would decrease or not and if they would be recovered 24-48 h after exhaustive exercise. The experimental end point was to complete 3 times of exhaustions or death from exhaustion. Circulating troponin I （cTnI） were detected from all of the experimental rabbits at rest [（0. 02±0. 01） ng/mL], which, in general, indicated that there existed a physiological release of troponin. After the first exhaustive swim, cTnI of the rabbits increased. However, with 24-hour rest, S1/S2, HR, and cTnI of the tested rabbits all returned toward baseline levels, which meant that the experimental rabbits experienced a cardiac fatigue process. After repeated exhaustion, overloading phenomena were observed, which led to death in 3 out of 11 rabbits, indicating their cardiac damage; the troponin elevation under this condition could be interpreted by pathological release. Evaluation of myocardial damage can not be based on the troponin levels alone, but can only be based on a comprehensive analysis.

关 键 词：心脏疲劳 心脏性死亡 肌钙蛋白 生理性释放 第1心音对第2心音幅值比值 

分 类 号：R87[医药卫生—运动医学]