硫化氢对急性肺损伤大鼠肺泡上皮细胞内质网应激的调节  

Regulatory effects of hydrogen sulfide on alveolar epithelial cell endoplasmic reticulum stress in rats with acute lung injury

作　　者：刘志伟[1] 王海英[1] 关岚[1] 赵斌[1] 

机构地区：[1]北京积水潭医院急诊科,北京100035

出　　处：《中华急诊医学杂志》2014年第8期876-881,共6页Chinese Journal of Emergency Medicine

摘　　要：目的 探索内源性硫化氢对于油酸诱导的急性肺损伤大鼠肺泡上皮细胞内质网应激的调节作用.方法 雄性Sprague Dawley大鼠随机（随机数字法）被分入正常对照组、油酸组、油酸＋硫氢化钠组及硫氢化钠对照组.每个组进一步分为2、4和6h3个时间点.对肺组织进行肺组织损伤半定量评分,检测肺组织湿、干质量比和匀浆硫化氢含量.通过免疫组化染色和蛋白印迹法检测内质网应激的标志蛋白（GRP78和elF2α）的表达.结果 肺损伤大鼠肺组织损伤半定量评分及湿、干质量比明显升高,而肺组织硫化氢含量,肺泡上皮细胞GRP78及eIF2α的表达明显降低.在硫氢化钠预处理的大鼠中,肺损伤半定量评分及肺组织湿、干质量比明显降低,而肺组织硫化氢含量,肺泡上皮细胞GRP78及eIF2α的表达明显升高.结论 内源性硫化氢可以通过促进肺泡上皮细胞内质网应激反应进而在肺损伤过程中起到保护作用.Objective To study the regulatory effect of hydrogen sulfide （H2S） on endoplasmic reticulum stress in alveolar epithelial cells of rats with acute lung injury （ALI） induced by oleic acid （OA）.Methods Seventy-two male Sprague Dawley （SD） rats were equally divided into control group （C group）,oleic acid-induced ALI group （OA group）,oleic acid-induced ALI with sodium hydrosulfide （NaHS） pretreatment group （OA ＋ NaHS group） and sodium hydrosulfide treatment group （NaHS group）.The model of acute lung injury was made by oleic acid intravenous injection in dose of 0.1 mL/kg.NaHS was injected intra-abdominally in dose of 1 ml/kg with concentration of 56 μmol/L 30 min before administration of oleic acid for pretreatment.In control groups,saline was used instead of oleic acid and NaHS in the equivalent volume.Six rats of each group were sacrificed at 2 h,4 h and 6 hours separately after modeling for observing the acute injury of lung tissue.Index of quantitative assessment of histological lung injury （IQA）,wet/dry weight ratio （W/D） and H2S level of lung tissues were measured.The endoplasmic reticulum stress markers included glucose-regulated protein 78 （GRP78） and α-subunit of eukaryotic translation initiation factor-2 （elF2α） in lung tissues were measured by immunohistochemical staining and Western blot.Results At the three observation intervals,the IQA score and W/D ratio of lung tissues significantly increased in rats-after OA injected,but significantly decreased in other rats receiving both OA and NaHS.At the three intervals,the level of H2S in lung tissue significantly decreased in rats after OA injected,but significantly increased in other rats receiving both OA and NaHS.GRP78 and elF2α decreased in rats after OA injected,but increased in other rats receiving both OA and NaHS especially at 4 h and 6 h after modeling.Conclusions The findings suggested that H2S could promote the response to alveolar epithelial cell endoplasmic reticulum stress in rats with ALI

关 键 词：硫化氢 急性肺损伤 内质网应激 

分 类 号：R563.8[医药卫生—呼吸系统]