乌司他丁对脓毒症大鼠脑组织的保护作用  

Protective effect of ulinastatin on cerebral tissue in septic rats

作　　者：曾朝涛[1] 张萌[1] 周天恩[1] 蒋龙元[1] 

机构地区：[1]中山大学附属孙逸仙纪念医院急诊医学科,广州510120

出　　处：《中华急诊医学杂志》2014年第8期885-889,共5页Chinese Journal of Emergency Medicine

基　　金：广东省自然科学基金（06021323）

摘　　要：目的 观察乌司他丁对脓毒症大鼠血脑屏障及脑细胞凋亡的影响.方法 清洁级雄性SD大鼠52只,随机（随机数字法）分为6组.假手术（Sham）6 h组、24h组,每组6只;脓毒症（CLP）及乌司他丁（UTI）6 h组、24h组,每组10只.Sham组只开腹后关腹,CLP组及UTI组用盲肠结扎穿孔法（CLP）建立脓毒症模型,UTI组建模后1h股静脉注射乌司他丁50 000U/kg,其他组注射等容量生理盐水.各组在建模后6h、24 h断脑取材,取材前进行神经功能缺损评分,取材后HE染色、称量脑干湿质量、伊文思蓝渗透法测定血脑屏障通透性,TUNEL免疫荧光检测脑细胞凋亡.应用SPSS 13.0软件统计学分析,多组比较采用方差分析,两两比较采用LSD-t检验.结果 UTI组大鼠神经功能评分低于Sham组（P＜0.05）,高于CLP组（P＜0.05）.光镜下CLP组大鼠神经细胞变性、坏死、水肿较UTI组明显.CLP 24 h组大鼠血脑屏障通透性较UTI 24 h组显著升高（P＜0.05）.CLP组大鼠脑细胞凋亡数较UTI组显著增多（P＜0.05）.结论 乌司他丁通过减轻血脑屏障损伤,抑制脑细胞凋亡,对脓毒症大鼠脑组织起保护作用.Objective To explore the effect of Ulinastatin on blood brain barrier （BBB） and apoptosis of neural cells in septic rats.Methods Fifty-two clean level male Sprague-Dawley rats were randomly （random number table） divided into six groups：Sham groups at 6 h and 24 h,each group with six rats.Sepsis groups （CLP） and Ulinastatin treated groups （UTI） at 6 h and 24 h,each group with ten rats.In CLP and UTI groups,cecal ligation and puncture （CLP） were performed to induce sepsis.Sham group was only opened and closed abdomen.Ulinastatin （50 000 U/kg） was administered via femoral vein 1 h after CLP.The same volume of saline instead of Ulinastatin was administered in Sham and CLP groups.The neurological status was assessed by Neurological Deficit Scale Scores （NDSS） at 6 h and 24 h after CLP.Then the brain was harvested for HE staining and weighing water content.The BBB permeability was assayed by Evans Blue dye extravasations.Apoptosis of neural cells were detected by TUNEL immune fluorescence.Statistical analysis was performed with SPSS version 13.0,ANOVA was used for multiple groups comparison and t-test for paired comparison.Results The Neurological Deficit Scale Scores of UTI group was lower than Sham group （P ＜ 0.05） but higher than that of CLP group （P ＜ 0.05）.Swelling,degeneration and edema were observed in cerebral cortex and hippocampal neurons in CLP group through light microscope,and were more serious than those in UTI group.Compared with UTI 24 h group,BBB permeability of CLP 24 h group significantly rose （P ＜ 0.05）.The number of apoptosis of neural cells increased more in CLP group than it did in UTI group （P ＜ 0.05）.Conclusions Ulinastatin could protect the cerebral tissue in septic rats by alleviating the damage of BBB and reducing the apoptosis of neural cells.

关 键 词：脓毒症 脑损伤 盲肠结扎穿孔 乌司他丁 伊文思蓝 血脑屏障 免疫荧光 凋亡 

分 类 号：R459.7[医药卫生—急诊医学]