针刺对大鼠三叉神经脊束核尾侧亚核慢性脑缺血性损伤神经元的保护作用  

Protective effects of acupuncture on cerebral chronic ischemia injured neurons in caudal spinal nucleus of spinal trigeminal nerve in rats

作　　者：郭森[1] 朱江[2] 孔祥玉[1] 赵淑敏[3] 

机构地区：[1]承德医学院脊髓损伤与修复研究室,承德067000 [2]承德医学院附属医院神经内科,承德067000 [3]承德医学院电子显微镜室,承德067000

出　　处：《解剖学杂志》2014年第4期500-503,516,共5页Chinese Journal of Anatomy

基　　金：河北省教育厅资助项目（QN20131075）;河北省卫生厅资助项目（20130340）

摘　　要：目的：探讨针刺对大鼠三叉神经脊束核尾侧亚核慢性脑缺血性损伤神经元的保护作用.方法：雄性Wistar大鼠,随机分成针刺组、缺血对照组、假手术组,针刺组大鼠电针刺激大鼠的“四白”穴,缺血对照组大鼠和假手术组大鼠,不予针刺处理.采用尼氏染色,计数三叉神经脊束核尾侧亚核内存活神经元的数目.电镜下观察神经细胞超微结构的变化以及有衣小泡的产生和形态.结果：光镜下,针刺组大鼠神经元计数明显高于缺血组,但仍低于假手术组.电镜下,缺血组大鼠神经元呈明显的缺血性改变,针刺组大鼠变化较轻,神经元内可见有衣小泡出现,假手术组神经元超微结构正常.结论：针刺“四白”穴对大鼠三叉神经脊束核尾侧亚核慢性脑缺血性损伤神经元有保护作用.Objective：To observe the protective effects of acupuncture on cerebral chronic ischemia injured neurons in the caudal spinal nucleus of the spinal trigeminal nerve in rats.Methods：Male Wistar rats were divided randomly into three groups：an acupuncture group,an ischemia group and a sham group.The acupuncture group was given the acupuncture at ＂Sibai acupoints＂ and the ischemia group and sham group were not.The neurons in caudal spinal trigeminal nucleus of trigeminal nerve were stained with Nissl dyeing method and counted.Then the morphological changes on the neurons and coated vesicles in caudal spinal trigeminal nucleus of trigeminal nerve were investigated under an electron microscope.Results：The number of the neurons in the acupuncture group was more than that in the ischemia group,but still less than that in the sham group.By electron microscopy,the neurons in the ischemia group had obvious ischemia changes.In the acupuncture group,the coated vesicles could be found,and the neurons had less changes.Conclusion：The forming of coated vesicles during acupuncture can act as the mechanism of protective function on the neurons injured by chronic ischemia in the caudal spinal nucleus of the trigeminal nerve in rats.

关 键 词：针刺 有衣小泡 脑缺血 三叉神经脊束核 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]