减压病兔脑与脊髓的TNF-α和GFAP变化  

Changes in levels of TNF-α and GFAP in brain and spinal cord of rabbits with decompression disease

作　　者：王翠翠[1] 刘晓红[1] 毛蕊琪[1] 曹永成[1] 毕利泉[1] 耿明[1] 

机构地区：[1]济南军区总医院病理科,济南250031

出　　处：《中华劳动卫生职业病杂志》2014年第8期607-609,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：全军医学科研“十二·五”面上项目资助课题（CWS11J241）

摘　　要：目的 探讨TNF-α、GFAP基因在减压病过程中的表达变化及作用机制.方法 成年健康雄性新西兰兔21只,随机分为正常组、减压病组、安全减压组,每组7只.采用快速减压方法制备减压病模型.借助光镜、荧光实时定量RT-PCR法和免疫组化方法观察脑和脊髓的病理形态学及TNF-α,GFAP基因mRNA和蛋白的表达变化.结果 减压病组脊髓白质内空泡形成,脊髓中TNF-α、GFAP基因mRNA和蛋白表达量明显增加,与正常对照组和安全减压组相比,差异有统计学意义（P＜0.01）;脑内减压病组、正常对照组和安全减压组之间TNF-α、GFAP基因mRNA和蛋白的表达量未见明显差异（P＞0.05）.结论 脊髓是减压病致中枢神经系统损伤的主要部位,减压病早期即可引起TNF-α和GFAP基因活化及相应蛋白大量表达,星形胶质细胞及促炎因子TNF-α在减压病脊髓损伤病变进程中发挥了重要作用.Objective To investigate the changes in expression of tumor necrosis factor-alpha （TNF-α） and glial fibrillary acidic protein （GFAP） in rabbits with decompression disease （DCS）,and to investigate the functioning mechanism.Methods A total of 21 healthy adult rabbits were randomly divided into 3 groups：normal control group,DCS group,and safe relief group,with 7 rabbits in each group.A rabbit DCS model was established by quick decompression.The changes in pathological morphology and mRNA and protein expression of TNF-α and GFAP in the brain and spinal cord of rabbits with DCS were determined by light microscopy,real-time PCR,and immunohistochemistry,respectively.Results Cavity formation was observed in the white matter of spinal cord in DCS group.The mRNA and protein expression of TNF-α and GFAP was significantly higher in the DCS group than in the normal control group and safe relief group （P〈0.01）,while no significant differences were observed in the brain （P〉0.05）.Conclusion Spinal cord is the main part of central nervous system injury in DCS.Activation of TNF-α and GFAP genes accompanied by increase in their protein expression can be observed at the early stage of DCS.The astrocytes and TNF-α play important roles in the process of spinal cord injury in DCS.

关 键 词：减压病 肿瘤坏死因子 胶质原纤维酸性蛋白 脊髓损伤 星形胶质细胞 

分 类 号：R845.21[医药卫生—航空、航天与航海医学]