人端粒酶反转录酶基因转染人胚胎大脑皮质神经元的凋亡  

Apoptosis of human embryonic cerebral cortex neurons transfected with human telomerase reverse transcriptase

作　　者：吴灵芝[1] 李水彬[1] 成钢卫[1] 汪华侨[2] 孔令平[3] 

机构地区：[1]中山大学附属梅州医院神经内二科,广东省梅州市514031 [2]中山大学中山医学院解剖学教研室脑研究室,广东省广州市510080 [3]广州医科大学卫生职业技术学院,广东省广州市510925

出　　处：《中国组织工程研究》2014年第29期4653-4657,共5页Chinese Journal of Tissue Engineering Research

基　　金：广东省教育厅科技创新项目(2012KJCX0089)~~

摘　　要：背景:人端粒酶反转录酶重组腺病毒转染原代培养的人胚胎大脑皮质神经元,可以促进细胞生存,抑制凋亡。目的:观察人端粒酶反转录酶对β淀粉样蛋白(amyloidβ-protein,Aβ)诱导人胚胎大脑皮质神经元凋亡的影响。方法:人胚胎大脑皮质神经元原代培养后,分为3组:对照组、Aβ25-35组和人端粒酶反转录酶组。Aβ25-35组和人端粒酶反转录酶组细胞在培养144 h后,用Aβ25-35 5μmol/L干预24 h。人端粒酶反转录酶组在Aβ25-35 5μmol/L干预72 h进行人端粒酶反转录酶基因转染。结果与结论:原代培养的人胚胎大脑皮质神经元培养7 d后,出现凋亡细胞,而Aβ25-35干预后使凋亡细胞数量增加,而人端粒酶反转录酶可防止Aβ25-35诱导的人胚胎大脑皮质神经元的凋亡。BACKGROUND： Human telomerase reverse transcriptase recombinant adenovirus trensfection could promote the survival and inhibit the apoptosis of primary cultured human embryonic cerebral cortex neurons. OBJECTIVE： To observe the effects of human telomerase reverse transcriptase on the apoptosis of human embryonic cerebral cortex neurons induced by amyloid ^-protein invention. METHODS： Primary cultured cerebral cortex neurons of human embryo were divided into three groups, namely control group, amyloid ^-protein （25-35） group, and human telomerese reverse trenscriptase group. Except the control group, the cells were cultured for 144 hours and intervened with 5 pmol/L amyloid ^-protein （25-35） for 24 hours. Furthermore, the cells in human telomerase reverse transcriptase group were transfected with human telomerase reverse trenscriptase group at 72 hours after intervention of 5 pmol/L amyloid 13-protein （25-35）. RESULTS AND CONCLUSION： After human embryonic cerebral cortex neurons were cultured for 7 days, the apoptotic cells were found in three groups. The apoptosis of neurons was obviously increased after intervention of amyloid ^-protein （25-35）. Human telomerese reverse transcriptase transfection could prevent the apoptosis of human embryonic cerebral cortex neurons induced by amyloid ^-protein （25-35）.

关 键 词：大脑皮质 细胞凋亡 端粒 端粒结合蛋白质类 转染 组织构建 组织工程 干细胞 胚胎干细胞 人端粒酶催化亚基 诱导 人胚胎大脑皮质神经元 阿尔茨海默病 凋亡 端粒酶 反转录酶 

分 类 号：R318[医药卫生—生物医学工程]