多孔磷酸钙骨水泥/纤维蛋白胶性状及对成骨细胞生物学行为的影响  

Mechanical and biological properties of porous calcium phosphate cement/fibrin glue for bone regeneration in vitro

作　　者：董静静[1] 毕龙[2] 李娜 郭誉 徐强[1] 雷伟[2] 崔赓[4] 

机构地区：[1]解放军空军临潼航空医学鉴定训练中心,陕西省西安市710600 [2]解放军第四军医大学西京医院骨科,陕西省西安市710032 [3]解放军兰州军区临潼疗养院疗一科,陕西省西安市710600 [4]解放军301医院骨科,北京市100039

出　　处：《中国组织工程研究》2014年第30期4831-4837,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年科学基金项目(81000790);北京市新星项目:2010年度北京市科技新星计划-崔赓(2010-77)~~

摘　　要：背景:在多孔材料磷酸钙骨水泥粉末中复合纤维蛋白胶是否能有效改善磷酸钙骨水泥的生物力学性能,同时加速其在体内的降解代谢,促进体内骨再生,目前还未被证实。目的:构建不同配比的多孔磷酸钙骨水泥/纤维蛋白胶复合材料,探索其诱导成骨的生物学特性。方法:将磷酸钙骨水泥与纤维蛋白胶分别以1∶1、3∶1、5∶1(mL/g)的配比复合,以单纯的磷酸钙骨水泥为对照,检测各组材料的初凝和终凝时间、压缩强度与弹性模量,扫描电镜观察材料表面结构。将第3代成骨细胞分别接种于4组材料表面,以单纯培养细胞为空白对照,观察不同时间点细胞黏附、增殖及碱性磷酸酶活性。结果与结论:1∶1、3∶1复合材料组初凝时间和终凝时间长于对照组(P<0.05),5∶1组复合材料组初凝时间和终凝时间短于对照组(P<0.05)。磷酸钙骨水泥/纤维蛋白胶复合材料表面较单纯的磷酸钙骨水泥致密而光滑,孔径随着纤维蛋白胶浓度的增加而降低。3∶1、5∶1复合材料组压缩强度高于对照组(P<0.05),1∶1、3∶1、5∶1复合材料组弹性模量高于对照组(P<0.05)。4组材料上的细胞黏附、增殖及碱性磷酸酶活性差异无显著性意义,但均高于空白对照组(P<0.05)。表明纤维蛋白胶的加入克服了磷酸钙骨水泥脆性较高的缺点,同时也保留了其促进成骨的作用。BACKGROUND： Fibrin glue introduced into calcium phosphate cement has not been confirmed whether this way could overcome the compressive limits and the low degradation of calcium phosphate cement and to modify the biological properties of calcium phosphate cement. OBJECTIVE： To explore the mechanical and biological properties of calcium phosphate cement/fibrin glue at different powder/liquid ratio for bone regeneration in vitro. METHODS： Calcium phosphate cement and fibrin glue were mixed at ratios of 1：1, 3：1, 5：1 （mL/g）, and the pure calcium phosphate cement served as controls. Setting time, scanning electron microscope and the biomechanical test were used to analyze the composite scaffold structure, physical performance and the mechanical properties. Passage 3 osteoblasts were respectively inoculated on the matedal surface of the four groups, and pure cellsserved as blank controls. Cell adhesion, proliferation and alkaline phosphatase activity were observed. RESULTS AND CONCLUSION： The initial and final setting time of calcium phosphate cement/fibrin glue at 1：1 and 3：1 （mL/g） was higher than that in the control group （P 〈 0.05）, while the initial and final setting time of calcium phosphate cement/fibrin glue at 5：1 （mUg） was lower than that of the control group （P 〈 0.05）. Scanning electron microscope showed smoother and denser surface of composite scaffolds compared with the pure calcium phosphate cement. The aperture of the composite scaffolds was decreased with the increasing concentration of fibrin glue. The compressive strength of composite scaffolds at 3：1 and 5：1 was higher than that of the control group （P 〈 0.05）, while the modulus of the composite scaffolds at 1：1, 3：1, 5：1 was higher than that of the control group （P 〈 0.05）. Cell adhesion, proliferation and alkaline phosphetase activity showed no difference among the three composite scaffold and control groups, but all higher than the blank control group （P 〈 0.05）. These findi

关 键 词：生物材料 骨生物材料 磷酸钙骨水泥 生物蛋白胶 骨缺损 生物力学 成骨细胞 增殖 碱性磷酸酶 骨再生 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]