运脾与化湿祛痰药物对饮食诱导肥胖大鼠肥胖程度及脂肪激素、瘦素抵抗的影响  

Effect of Pi Transportation,Dampness Resolving and Phlegm Expelling Herbs on the Obesity Degree,Fat Hormones,and Leptin Resistance in Diet-induced Obesity Rats

作　　者：姜萍[1] 姜月华[2] 

机构地区：[1]山东中医药大学中医内科学教研室,济南250355 [2]山东中医药大学附属医院中心实验室,济南250011

出　　处：《中国中西医结合杂志》2014年第8期997-1002,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81102676)

摘　　要：目的观察运脾与化湿祛痰药物对饮食诱导肥胖(diet-induced obesity,DIO)大鼠肥胖程度及脂肪激素、瘦素抵抗的影响。方法 Wister大鼠120只,10只作为空白对照组,给予基础饲料,其余110只给予高脂高营养饲料17周,按照体重,得到DIO大鼠40只和肥胖抵抗(diet-in duced obesity resistance,DIOR)大鼠10只,将DIO大鼠又分为DIO模型组、西布曲明组、化湿祛痰组、运脾组4组,每组10只,分别以生理盐水(2 mL/d)、西布曲明(每天1.6 mg/kg)、化湿祛痰药物(每天3.2 g/kg)、运脾药物(每天3.2 g/kg)灌胃,空白对照组与DIO-R组每天予生理盐水(2 mL)灌胃。灌胃期间空白对照组予基础饲料,余5组继续高脂饲料。灌胃16周后,测量体重、身长,取腹腔内全部脂肪,测量肥胖程度及脂肪含量。取血测定胰岛素抵抗指数(insulin resistance index,IRI)、血糖、甘油三酯、胆固醇、瘦素、神经肽Y(neuropeptide Y,NPY)、肿瘤坏死因子(TNF-α)、脂联素(adiponectin)。取脂肪匀浆测定瘦素、TNF-α、脂联素、细胞因子转录负调节因子(suppressor of cytokine signaling-3,SOCS-3)等有关脂肪激素和炎症因子。结果与正常大鼠比较,DIO大鼠体重、体重指数、脂肪系数、IRI、血清瘦素、TNF-α、SOCS-3水平均明显升高(P<0.05,P<0.01),血清NPY和脂肪瘦素、脂联素下降(P<0.05);DIO-R大鼠瘦素升高、NPY降低;与DIO组比较,DIO-R组大鼠体重、体重指数、脂肪系数、IRI、血清NPY、TNF-α、SOCS-3均降低(P<0.05,P<0.01),血清脂肪瘦素、脂联素均升高(P<0.05,P<0.01)。经药物干预治疗后,西布曲明组大鼠体重、体重指数、脂肪系数、脂肪匀浆TNF-α明显降低(P<0.05,P<0.01);化湿祛痰组血清TNF-α降低,脂肪瘦素、脂联素升高(P<0.05),虽体重指数、脂肪系数、IRI、血清瘦素和NPY、SOCS-3有下降趋势,但差异无统计学意义(P>0.05);运脾组体重、体重指数、脂肪系数、IRI、TNF-α、SOCS-3、TNF-α均降低(P<0.05,P<0.01);血清和脂Objective To observe the effect of Pi transportation, dampness resolving and phlegm expelling herbs （PTDRPEH） on the obesity degree, fat hormones, and leptin resistance in diet-in- duced obesity （DIO） rats. Methods Among the 120 Wister rats, 10 were recruited as the blank control group （fed with basal forage）, and the remaining 110 were administered with high-fat high-nutrition forage for 17 weeks. According to weight, we obtained 40 DIO rats and 10 diet-induced obesity resistance （DIO- R） rats. DIO rats were further divided into four groups, i.e., the DIO model group （normal saline, at thedaily dose of 2 mL）, the sibutramine group （at the daily dose of 1.6 mg/kg）, the dampness resolving and phlegm expelling group （DRPE, at the daily dose of 3.2 g/kg）, and the Pi transportation group （PT, at the daily dose of 3.2 g/kg）. All were given by gastrogavage. Normal saline （2 mL） was given by gas- trogavage to rats in the blank control group and the DIO-R group. The basal forage was administered to rats in the blank control group, while high fat forage was continually given to rats in the remaining five groups. Their body weights and body lengths were measured after 16 weeks of gastrogavage. All intra-ab- dominal fat was taken out to measure the degree of obesity and fat contents. Insulin resistance index （IRI）, blood glucose, triglycerides, cholesterol, leptin, neuropeptide Y （NPY）, tumor necrosis factor α （TNF-α）, and adiponectin were detected after blood withdrawing. Leptin, TNF-α, adiponectin, suppres- sors of cytokine signaling-3 （SOCS-3）, and other relevant adipose hormones and inflammatory cytokines were examined in the fat homogenate. Results Compared with the blank control group, DIO model rats＇ body weight, body mass index （BMI）, fat factor, IRI, serum leptin, TNF-α, and SOCS-3 significantly in- creased （P 〈0.05, P 〈0.01 ） ; serum NPY, serum leptin, and adiponectin decreased （P 〈0.05）. Leptin increased and NPY decreased in DIO-R model r

关 键 词：饮食诱导肥胖 瘦素抵抗 细胞因子转录负调节因子 运脾 化湿祛痰 

分 类 号：R285.5[医药卫生—中药学]