白细胞介素-1β在过敏性鼻炎小鼠的表达及其对鼻上皮细胞肿瘤坏死因子α诱导蛋白2的影响  

Expression of interleukin-1β in the nasal epithelial cells of the allergic rhinitis mouse and its effect on the production of tumor necrosis factor-α-inducible protein-2 in the nasal epithelial cells

作　　者：王敏[1] 王向东[1] 李颖[1] 范尔钟[1] 杨军[1] 张罗[1] 

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京市耳鼻咽喉科研究所,耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),鼻病研究北京市重点实验室

出　　处：《中国耳鼻咽喉头颈外科》2014年第6期315-318,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家杰出青年科学基金(81025007);教育部"长江学者和创新团队发展计划"(IRT13082)联合资助

摘　　要：目的探讨白细胞介素1β(IL-1β)在过敏性鼻炎(allergic rhinitis,AR)小鼠鼻黏膜的表达及其体外对鼻上皮细胞肿瘤坏死因子α诱导蛋白2(tumor necrosis factor-α-inducible protein-2,TNFAIP2)表达的影响。方法建立AR小鼠模型,检测鼻黏膜IL-1β基因的表达;培养小鼠鼻原代上皮细胞,用不同剂量IL-1β刺激,检测上皮细胞中TNFAIP2基因和蛋白的表达。结果 AR小鼠鼻黏膜IL-1β基因表达相对值为8.7±1.74,显著高于对照组1.21±0.26(P=0.0017);小鼠鼻上皮细胞经25、50 ng/ml IL-1β刺激后TNFAIP2基因表达的相对值分别为15.83±1.65、26.50±4.25,均显著高于对照组2.31±0.78(P<0.0001,P=0.0002),且50 ng/ml刺激组显著高于25 ng/ml刺激组(P=0.04);TNFAIP2蛋白表达呈相同趋势。结论 AR小鼠鼻黏膜IL-1β表达增强,IL-1β体外能够诱导鼻上皮细胞表达TNFAIP2,提示AR中IL-1β可能通过调节TNFAIP2的表达发挥作用。OBJECTIVE To investigate the expression of interleukin-lβ （IL-lβ） in the nasal mucosa of the allergic rhinitis （AR） mouse and the effect of IL-Iβ on tumor necrosis factor-a-inducible protein-2 （TNFAIP2） expression in primary nasal epithelial cells in vitro. METHODS AR mouse model was developed and 1L-1β） gene expression in the nasal mucosa was measured. Primary epithelial cells were cultured from mouse nasal tissue and stimulated with different concentration of IL-1β in vitro, the expression of TNFAIP2 gene and protein was measured.

关 键 词：鼻炎 变应性 常年性 白细胞介素lβ 鼻黏膜 上皮细胞 肿瘤坏死因子α诱导蛋白2 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]