预定位技术用于超声分子成像的体外实验  

Study on pretargeting technology for ultrasound molecular imaging in vitro

作　　者：张炜阳[1] 黄晓玲[1] 王志刚[2] 张勇[1] 李茂萍[1] 

机构地区：[1]重庆医科大学附属第一医院超声科,重庆400016 [2]重庆医科大学超声影像学研究所,重庆400016

出　　处：《中国医学影像技术》2014年第8期1145-1150,共6页Chinese Journal of Medical Imaging Technology

基　　金：国家临床重点专科建设项目(国卫医办函[2013]544号)

摘　　要：目的探讨预定位技术提高超声造影剂靶向肿瘤细胞的能力。方法采用两步法预定位技术靶向SKOV-3卵巢癌细胞:第一步加入生物素化的CEA抗体,第二步加入结合链霉亲和素的造影剂。结果制备的结合链霉亲和素的PLGA造影剂平均粒径为(346.20±74.49)nm。链霉亲和素与造影剂的连接效率为(95.02±0.62)%。预定位靶向组细胞结合的造影剂明显多于其余各组。结论预定位技术能提高造影剂的靶向性,可提高超声分子成像的敏感性。Objective To evaluate pretargeting technology for improving the ability of targeting tumor cells.MethodsPoly（lactic-co-glycolic acid）nanoparticles（NPs）were prepared by double-emulsion method.Streptavidin was covalently bound to the surface of NPs through a carbodiimide reaction.A pretargeting approaches had been studied,first step was added in biotinylated antibodies,and second step was added in streptavidin coated poly（lactic-co-glycolic acid）nanoparticles（SA-NPs）.The targeting efficacy of SA-NPs toward SKOV3 cells were determined by laser scanning confocal microscope and flow cytometry.Results The mean size of SA-NPs was（346.20±74.49）nm.The effective binding between NPs and streptavidin was（95.02±0.62）%.In pretargeting group,the number of nanoparticles conjugated to SKOV-3cells was significantly more than the other group.Conclusion These results indicate that the efficacy of NPs targeting to tumor cells can be increased by this pretargeting method.

关 键 词：体外 超声检查 分子成像 

分 类 号：R445.1[医药卫生—影像医学与核医学]