银杏内酯B对动脉粥样硬化大鼠炎症细胞因子的影响  

Effects of Ginkgolide B on Inflammatory Cytokines in Rats with Atherosclerosis

作　　者：刘艳茹 锁建军 雷如意 王长娥[1] 张博爱[1] 

机构地区：[1]郑州大学第一附属医院神经内科,450052 [2]河南省三门峡市第三人民医院神经内科,472143

出　　处：《医学研究杂志》2014年第8期131-133,共3页Journal of Medical Research

摘　　要：目的探讨银杏内酯B对动脉粥样硬化(AS)大鼠血清炎症细胞因子的影响。方法采用高脂饲料喂养和维生素D3腹腔注射的方法建立大鼠AS模型,分为正常组、模型组、阳性组(辛伐他汀5mg/kg)、银杏内酯B 3个剂量组(8、4、2mg/kg),每组10只。灌胃给药4周后,各组取主动脉,光镜下观察病理改变,进行病变分级评分;取血清,ELISA法测定TNF-α、IL-1和IL-6水平。结果银杏内酯B能改善AS大鼠的主动脉病变,高剂量组与模型组比较差异有统计学意义(P<0.05)。银杏内酯B各剂量可明显降低血清TNF-α和IL-6水平,与模型组比较差异均有统计学意义(P<0.01)。银杏内酯B高、中剂量可降低血清IL-1水平,与模型组比较差异有统计学意义(P<0.05)。结论银杏内酯B对大鼠AS具有一定的治疗作用,可能与抑制炎症因子的表达有关。Objective To investigate the effects of ginkgolide B （GB） on inflammatory cytokines in rats with atherosclerosis （AS）.Methods Rats model of AS was established by administration of high-fat diet and vitamin D3 （i.p.）.Rats were divided into 6 groups （n =10） at random,namely normal,model,positive （simvastatin,5mg/kg） and GB high,medium and low dose groups （8,4 and 2mg/kg,respectively）.After 4 weeks of oral treatment,the aortic pathologic changes were observed under microscope and graded.The sera of all rats were collected to determine the levels of TNF-α,IL-1 and IL-6 by ELISA.Results Administration of GB improved the severity of aortic pathology in AS rats,and the pathologic grade of GB high dose group showed a significant difference compared with that of model group （P ＜ 0.05）.GB （8,4 and 2mg/kg） markedly decreased the TNF-α and IL-6 levels in serum of AS rats （P ＜ 0.01）.Meanwhile,the levels of IL-1 in GB （8 and 4mg/kg） groups were significantly lowered than the model group （P ＜ 0.05）.Conclusion GB shows certain therapeutic effect on AS rats,and the mechanisms may be involved in inhibiting the expression of inflammatory cytokines.

关 键 词：银杏内酯B 动脉粥样硬化 TNF-Α IL-1 IL-6 

分 类 号：R541[医药卫生—心血管疾病]