等位基因特异性PCR技术及其法医学应用  

Allele-specific PCR and Its Application in Forensic Science

作　　者：聂燕钗[1,2] 王斌[3] 赵子琴[1] 周怀谷[2] 

机构地区：[1]复旦大学上海医学院法医学系,上海200032 [2]上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室,上海200083 [3]上海市公安局浦东分局刑事科学技术研究所,上海200135

出　　处：《法医学杂志》2014年第4期282-287,共6页Journal of Forensic Medicine

基　　金：公安部科技基础工作专项资助项目(2011GABJC006)

摘　　要：等位基因特异性PCR(allele-specific polymerase chain reaction,AS-PCR)是一种基于等位基因特异性引物引导的PCR技术,可以有效地分析单核苷酸多态性(SNP),包括碱基的转换、颠换以及插入/缺失多态性,在疾病研究、分子诊断以及法医物证学研究中具有很好的应用价值。本文系统地综述了AS-PCR技术的原理、检测手段、改进方法及在常染色体、Y染色体和线粒体SNP等领域的研究成果,探讨其法医学应用价值。Allele-specific polymerase chain reaction （AS-PCR） is a technique based on allele-specltlcprimers, which can be used to analyze single nucleotide polymorphism （SNP） effectively including thetransition, transversion and insertion/deletion polymorphism and has been exploited in the study of dis-eases research, molecular diagnosis, and forensic biological evidence. The article systematically reviewsthe principle, the detection methods, improvement of AS-PCR, and its research updates in the fields ofautosome, Y chromosome and mitochondrial SNP, as well as its application in forensic science.

关 键 词：法医遗传学 聚合酶链反应 综述[文献类型] 等位基因特异性 

分 类 号：DF795.2[医药卫生—法医学]