细柄蕈树嫩枝扦插生根过程中内源激素动态特性研究  

The endogenous hormones' variation during the adventitious root formation of Altingia gracilipes cuttings

作　　者：范俊俊[1] 张往祥[1] 蔡喜彦 赵明明[1] 魏宏亮[1] 

机构地区：[1]南京林业大学林学院,江苏南京210037

出　　处：《江苏林业科技》2014年第4期11-14,共4页Journal of Jiangsu Forestry Science & Technology

基　　金：江苏省农业科技自主创新项目:"枫香亚科耐寒常绿阔叶行道树新品种选育"CX(11)1040

摘　　要：以3年生细柄蕈树实生苗嫩枝为试验材料,运用酶联免疫吸附测定法(ELISA),研究其在扦插生根过程中内源激素含量的变化,旨在揭示细柄蕈树扦插生根的机理。结果表明,与CK相比,以质量浓度300 mg/L的NAA处理插穗,能有效缩短其生根周期;扦插生根过程中,NAA处理与CK的几种内源激素(IAA、ZT和ABA)含量随时间变化的趋势相同,均呈正"V"形变化;NAA处理可调节内源激素,促进不定根的形成和发育;不定根形成期间,NAA处理与CK的IAA/ZT、IAA/GA3和IAA/ABA谷值的出现时间(20 d和30 d)与愈伤组织形成期重合,内源激素的这种节奏性变化反映了生根与生理之间的协同性。To reveal the rooting mechanism of Altingia gracilipes,endogenous hormone dynamics in the 3-year-old softwood cuttings during the adventitious root formation was studied by ELISA. The result showed that rooting cycle of the cuttings treated with 300 mg/L NAA was clearly shortened compared with that of CK. The change trend of the endogenous hormones（IAA,ZT and ABA） by NAA treatment was parallel to that of CK,which was featured with＂V-shape＂. Meanwhile,indications of synergetic effects were observed between endogenous and exogenous hormones. During the adventitious root formation,the valley in the curves of IAA/ZT,IAA/GA3 and IAA/ABA with the NAA treatment and CK appeared on the 20 thand 30threspectively,presenting synergetic effect along with root formation.

关 键 词：细柄蕈树 扦插 内源激素 

分 类 号：Q949.751.4[生物学—植物学]