药用植物珠子参新鲜块根DNA提取方法研究  

Comparision among Four Methods of DNA Extraction from Chinese Herbal Medicine which Panacis majoris

作　　者：杨维泽[1] 史云东[2] 许宗亮[1] 杨绍兵[1] 张金渝[1] 

机构地区：[1]云南省农业科学院药用植物研究所,云南昆明650231 [2]玉溪师范学院化学与环境科学系,云南玉溪653100

出　　处：《云南中医学院学报》2014年第4期25-28,共4页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81260610);国家农业部公益性行业专项(201303117)

摘　　要：目的为获取珠子参高质量、高产量的总DNA,满足SSR-PCR标记实验。方法采用经典CTAB法、改良CTAB法、高盐低PH法和SDS法对珠子参总DNA提取方法进行比较研究,用紫外分光光度法和琼脂糖凝胶电泳检测DNA质量。结果 4种方法中产量最高的为经典CTAB法,产率为149.533ng/g,纯度最高的为改良CTAB法,OD260/OD280为1.796。结论 4种方法提取的DNA质量均满足SSR-PCR标记实验,扩增效果改良CTAB法和SDS法效果最佳。Objective To get the high quality and high yield of total DNA from Panacis majoris. Methods Used the methods CTAB,improved CTAB,SDS and high salt low PH extraction the total DNA,The quality of the DNA was examined with spectrophotometer and agarose gel electrophocesis. Results The results showed that the highest yield was CTAB,productive rate is149. 533ng/g,the best quality is improved CTAB,OD260/OD280 IS 1. 796. Conclusion The total DNA from the four extraction methods,all can used the SSR marker,the best effect was improved CTAB and the SDS.

关 键 词：珠子参 DNA提取 SSR扩增 

分 类 号：R284.2[医药卫生—中药学]