肌浆网钙ATP酶过表达对心房颤动兔心房心电生理的影响  

Effect of sarcoplasmic reticulum Ca^(2+)-ATPase2a overexpression on cardiac electrophysiology in rabbit with atrial fibrillation

作　　者：王红丽[1] 李志强[1] 周贤惠[2] 周祁娜[2] 汤宝鹏[2] 

机构地区：[1]新疆医科大学第一附属医院临床医学研究院,乌鲁木齐830054 [2]新疆医科大学第一附属医院心脏起搏电生理科,乌鲁木齐830054

出　　处：《中华临床医师杂志（电子版）》2014年第16期60-63,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：新疆维吾尔自治区自然科学基金(2014211C066)

摘　　要：目的探讨肌浆网钙ATP酶2a(SERCA2a)过表达对急性心房颤动(AF)兔心房肌电重构及结构重构的影响。方法 36只成年新西兰大白兔,随机分为假手术组作为对照组(Control组,n=12)、腺病毒/绿色荧光蛋白+AF组(rAd/GFP+AF组,n=12)、腺病毒/SERCA2a+绿色荧光蛋白+AF组(rAd/SERCA2a/GFP+AF组,n=12)。采用快速心房起搏(频率600次/min)制作急性AF模型。观察各组起搏0、4、8、12、24 h心房有效不应期的变化;应用超声测量左右心房内径、左心室射血分数;荧光显微镜下观察心肌组织绿色荧光表达情况。结果 rAd/SERCA2a/GFP+AF组自起搏12 h后AERP较rAd/GFP+AF组延长(P<0.05)。rAd/SERCA2a/GFP+AF组与rAd/GFP+AF组相比较,兔左右心房直径增大,但增加不显著(P>0.05);EF值升高,两组有统计学差异(P<0.05)。rAd/SERCA2a/GFP+AF组兔心肌冰冻切片在荧光显微镜下可见弥漫绿色荧光。结论以rAd为载体介导SERCA2a基因转导在心房肌组织中过表达可以抗快速起搏引起的AERP缩短,可有效逆转快速心房起搏兔心房肌的电重构及结构重构,有望成为一种潜在的治疗AF的方法。ObjectiveTo explore the effects of sarcoplasmic reticulum calcium ATPase2a （SERCA2a） gene on atrial electrical remodeling and structure remodeling in atrial fibrillation （AF） rabbits by 24 h rapid atrial pacing.MethodsThirty-six New Zealand rabbits were randomized into three groups （n=12）, including control group, rAd/GFP＋AF group and rAd/SERCA2a/GFP＋AF group, AF was induced by rapid atrial pacing （600 beats/min） for 24 hours in each animal of rAd/GFP＋AF group and rAd/SERCA2a/GFP＋AF group, each animal of control group was not paced. The changes of atrial effective refractory period （AERP） were observed 0, 4, 8, 12, 16 and 24 hours after pacing. Left and right atrial diameter and left ven-tricular ejection fraction （EF） were examined by cardiac ultrasonography. Animals were scarified to observe expression of the green fluorescent protein in the myocardial frozen section of rabbits in rAd/SERCA2a/GFP＋AF group under the inverted fluorescence microscope. ResultsAERP was markedly prolonged in rAd/SERCA2a/GFP＋AF group compared with rAd/GFP＋AF group after pacing for 12 hours（P〈0.05）. Compared with rAd/GFP＋AF group, the left and right atrial volume of rabbits in rAd/SERCA2a/GFP＋AF group were enlarged but the increase was not significant （P〉0.05）, left ven-tricular ejection fraction （EF） was increased significantly （P〈0.05）; Under the fluorescence microscope, widespread green fluorescence was observed in the myocardial frozen section of rabbits in rAd/SERCA2a/GFP＋AF group.ConclusionThese results support the hypothesis that overexpression of SERCA2a is able to reverse the atrial electrophysiologic remodeling and structure remodeling in AF rabbits induced by rapid atrial pacing.It is expected to become a potential method for the treatment of AF.

关 键 词：钙转运ATP酶类 心房颤动 电重构 结构重构 

分 类 号：R541.75[医药卫生—心血管疾病]