基于四交群体的连锁图谱构建(英文)  

Constructing linkage map based on a four-way cross population

作　　者：蒋蓓蓓[1] 余世洲[1] 肖炳光[2] 楼向阳[3] 徐海明[1,4] 

机构地区：[1]浙江大学农业与生物技术学院生物信息学研究所,杭州310058 [2]云南省烟草农业科学研究院,昆明650031 [3]阿拉巴马大学伯明翰分校生物统计系 [4]浙江大学空气污染与健康研究中心,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2014年第4期387-396,共10页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：Supported by the National Natural Science Foundation of China(No.31271608);the Yunnan Provincial Tobacco Company Project(No.08A05);the CNTC Science and Technology Project(No.110200701023);the National Institutes of Health(No.DA025095)

摘　　要：目前,遗传连锁图谱构建主要基于两纯系亲本交配的衍生群体,存在遗传多样性不足等局限,不适合于烟草等基因组多样性较低的物种,因为往往没有足够的多态性标记可用于图谱的构图,稀疏的标记图谱很难有效用于标记辅助选择及基因定位.本研究提出了基于四交群体的遗传图谱构建方法,通过计算机模拟验证了该方法的可行性和有效性,研制了配套的计算机分析程序.该方法的主要步骤及特点如下:1)基于最大似然法估计成对标记间的遗传距离;2)基于标记距离矩阵进行聚类分析,将标记分成不同的类群(连锁群);3)对每一连锁群,先确定连锁群两端的标记,再通过3标记间遗传距离的比较,将其他标记逐步插入到合适的排列位置,最终确定整个连锁群的标记顺序.蒙特卡洛模拟证实了该方法可行、有效,且适用于其他2个自交系亲本交配的衍生群体.Currently , developing genetic linkage map mostly use the derived-populations from crossing of two homogenous parents , which only covers limited genetic diversity and is inappropriate for some species , such as tobacco with lower diversity in genome . It is very general that there are no sufficient polymorphic markers to construct linkage map and ineffective to conduct marker-assisted selection （ M AS ） and quantitative trait locus （ QTL） mapping based on lower density linkage map . This study proposed a method for developing genetic linkage map based on a four-way cross population . Computer simulation was conducted to investigate the feasibility and effectiveness of the method and a supporting program was designed . The main procedures and features of the proposed method were summarized as follows： 1） estimating genetic distance of any paired markers based on maximum likelihood method;2） splitting all markers into different groups （ linkage group） by cluster analysis based on genetic distance of markers; 3） for each linkage group , two end markers were first determined , then the marker order could be determined by inserting other markers in appropriate position by distance analysis of any three neighboring markers . Monte Carlo simulation showed that the proposed method is feasible , effective , and applicable in other derived populations from crossing of two homogenous parents .

关 键 词：四交群体 遗传距离 遗传图谱 连锁分析 蒙特卡洛模拟 

分 类 号：Q348[生物学—遗传学]